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学号: 4109014048 泰山医学院毕业设计（论文） 题目： 蜂胶黄酮抗H2O2诱导PC12细胞 凋亡机制的研究 院（部）系 药学院 所 学 专 业 药学 年级、班级 完成人姓名 指导教师姓名 专业技术职称 2013年 6 月 18 日 论文原创性保证书 我保证所提交的论文都是自己独立完成，如有抄袭、剽窃、雷同等现象，愿承担相应后果，接受学校的处理。 专业： 班级： 签名： 20 年 月 日 摘 要 组，、、H2O2（140μmol/L）24h诱导过氧化损伤。 H2O2组与空白对照组比较染色明显加深,而蜂胶黄酮组染色明显变浅，说明蜂胶黄酮能对抗H2O2诱导细胞凋亡；周期结果显示H2O2组处于G0/G1细胞明显增多，而处于G2/M、S期细胞明显减少，细胞增殖降低，而蜂胶黄酮增加S期细胞促进细胞增殖；与空白对照组相比H2O2组活性氧水平、细胞内Caspase-3含量明显增高，而蜂胶黄酮各剂量组活性氧水平降低，Caspase-3含量减少。 结论 蜂胶黄酮对H2O2诱发细胞有显著的作用。 Abstract Objective:?To observe the protection of?propolis flavonoids?on rat with injuried pheochromocytomacells(PC12) induced by??hydrogen peroxide(H2O2)?and to explore its possible mechanism. Methods:???Culture?PC12 cells,the cells in?the logarithmic growth phase were?divided into five groups,blank control group,model group,propolis flavone?of high,medium and low dose group,the dose of 200mg/L,100 mg/L,50 mg/L.?After 2h 0f?Pharmacological preconditioning,the cells?were incubated with H2O2(140 μ mol/L)?for?24h?to induce?oxidative damage.?TUNEL Kit?is used for?determining Cell apoptosis,Flow cytometry was used to detect the intracellular ROS level and cell cycle,ELISA is used for determining the concentration of protein Caspase-3 in cells. Results:?Apoptosis of TUNEL cells staining, H2O2 group compared with the control group, staining was deepened, and the propolis flavone group was significantly lighter, that propolis flavonoids can antagonize the apoptosis induced by H2O2 Cycle showed that H2O2 group in G0/G1 cells were increased, and in the G2/M,S phase cells decreased significantly, reduced cell proliferation, and propolis flavonoids increased S phase cells promoting cell proliferation; compared with the blank control group, caspase-3 in H2O2group, the levels of reactive oxygen species in cells increased significantly, while the p