草履虫论文(开放实验室).docVIP

淮北师范大学 2010届学士学位论文 草履虫的采集与培养 学院、专业 生命科学学院 2006级生物工程 研 究 方 向 动 物 学 学 生 姓 名 朱 力 力 学 号 06154304071 指导教师姓名 朱 艳 芳 指导教师职称 讲 师 2010年 5 月 25日  草履虫的采集与培养 朱力力 （淮北师范大学生命科学学院） （指导老师：朱艳芳） 摘 要：本文草履虫的采集纯化培养，及不同的温度、pH培养液。实验结果显示草履虫在不同温度条件下生长繁殖速度不同，15℃不适合用于快速繁殖，但可用于草履虫形态观察的培养；25℃可用于草履虫的中长期培养；35℃可以用于草履虫的快速繁殖。在９种培养液中第９号培养液即河水稻草煮液+米饭粒最有利于草履虫的生长繁殖 pH值为70左右时草履虫的生长速度最快，且峰值期维持的时间最长。总结出在现有条件下采集和培养草履虫的简捷方法，为实验室用草履虫培养提供相关参考以便于实验教学与研究 关键词：草履虫；采集；培养  Collection and Cultivation Paramecium caudatum Zhu Li-li （School of Life Sciences，Huaibei Normal University） (Tutored by Zhu Yan-fang) Abstract This contained the collection and purifying culture of Paramecium caudatum . Different temperature ,pH level and medium were also studied. The results showed that in different temperature the Paramecium caudatum grew differently . 15 ℃ was not suitable for rapid propagation , but could be used as morphological observation temperature ; 25℃ could be used for mid-long term cultivation ; 35℃ was suitable for rapid propagation . The ninth medium , boiled water from the river with straw and rice , was the best in the nine cultivation medium for the Paramecium caudatum rapid propagation . Around the 7.0 pH level was the optimum level, the number of Paramecium caudatum was earlyer reaching to the pick and the duration of peak was the longest in the three pH levels. This paper summarized the simple and effective method of Paramecium caudatum cultivation , and offered the material to experimental teaching and related research . Key words 　Paramecium caudatum；Collection ；Cultivation 目录 引言 1 1 材料和方法 1 1．1 材料 1 1．2 方法 1 1．2．1 草履虫的采集 1 1．2．2 草履虫的扩大培养 1 1．2．3 培养液的配制 2 1．2．4 草履虫的纯化 3 1．2．5 温度、培养液及pH值对草履虫的影响 3 1．2．5．1 温度对草履虫的影响 3 1．2．5．2 培养液对草履虫的影响 3 1．2．5．3 pH值对草履虫的影响 3 1．2．6 草履虫的计数 4 2． 结果和讨论 4 2．1