大肠杆菌抗氧化应激反应的蛋白质组学分析.docVIP

生命科学学院 本科毕业论文 题目：大肠杆菌抗氧化应激反应的蛋白质组学分析 专 业： 生 物 技 术 班 级： 07（1）班 姓 名： 王芳 指导教师： 潘建义 学 号： 入学年月： 2007年9月 选题来源： 纵向课题 报告日期 2011年05月24日 浙江理工大学本科毕业论文诚信声明 本人郑重声明：我恪守学术道德，崇尚严谨学风。所呈交的毕业论文，是本人在导师的指导下，独立进行研究工作所取得的成果。除文中已明确注明和引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的作品及成果的内容。论文为本人亲自撰写，我对所写的内容负责，并完全意识到本声明的法律结果由本人承担。 论文作者签名： 日期： 年 月 日 摘 要 细胞代谢过程中，部分电子逃逸出氧化还原系统以氧分子作为电子受体，产生具有毒害作用的高能量活性氧分子（reactive oxygen species，ROS）包括超氧阴离子、单线态氧、、羟自由基脂质过氧化的中间产物。活性氧是一种广谱杀菌剂，是宿主抗菌作用的有效方式。但细菌在长期的进化发展出多种对策来抵抗宿主杀菌作用为检测与此相关的蛋白质，本研究主要通过正常及氧化应激条件下对大肠杆菌的培养，差速离心法提取膜蛋白SDS，基质辅助激光解吸电离飞行时间串联质谱(MALDI-TOF-MS/MS)进行鉴定分析。麦芽糖孔蛋。 关键词活性氧氧化应激反应；MALDI-TOF ABSTRACT During the metabolism in the cell, oxygen molecules as an important electron acceptor, Also accompanied by some electronic escape from the redox system, produce some high energy reactive oxygen species which has poisoned, including superoxide anion (O2-), singlet oxygen, hydrogen peroxide, hydroxyl radical, also includes the intermediate products of lipid metabolism. Reactive oxygen species (ROS) is a broad-spectrum fungicide. It is one of the effective way to host cell antimicrobial. However, bacteria in the long-term evolution development of a variety of strategies to resist the host immune bactericidal action. To detect which proteins associated with this system, in this study, mainly through training two group of the pathogenic E. coli, the one Treatment by hydrogen peroxide, the other one as the control group, Broken cells by ultrasound. Extraction the membrane proteins by differential centrifugation, make a SDSelectrophoresis with the Protein, Digging the significant difference protein band, after gel digestion, then identified by MALDI-TOF-MS/MS. We found two proteins which have significant difference, they are Maltoporin and outer membrane protein A (OmpA). Maltoporin and OmpA are associate to the small molecules