丝瓜种质资源亲缘关系的SRAP分析.pdfVIP

植物遗传资源学报2012,13(6):1061-1066 Journal of Plant Genetic Resources 丝瓜种质资源亲缘关系的SRAP分析 1 1 1 1 2 1 崔竣杰 ,宋建文 ,汪国平 ,林明宝 ,李连芳 ,胡开林 1 2 ( 华南农业大学园艺学院,广州510642; 广州市农业科学院,广州510308) 　 　 摘要:利用SRAP标记,对来源不同的56份有棱丝瓜和8份无棱丝瓜种质的亲缘关系进行了分析。 从 144 对SRAP 引物 中筛选出60 对多态性强、重复性好的SRAP 引物。 共扩增得到1433条谱带,其中多态性谱带1280条,平均每对引物扩增得到 21．33条多态性谱带,多态性位点百分率为88．74%。 将丝瓜种质利用UPMGA方法进行聚类分析,结果表明,64 份丝瓜种质 的遗传相似系数为0．17~0．98。 在遗传相似系数0．17 处,供试种质被分为2 大类群,第一大类群为普通丝瓜,第二大类群为 有棱丝瓜,在第二大类群中又被分为以长绿型丝瓜为主和短果型丝瓜2 亚群。 丝瓜类群的划分与形态学性状比较一致,即首 先与棱沟的有无密切相关,其次与瓜条的长短、颜色有较高的相关性。 　 　 关键词:丝瓜;种质资源;亲缘关系;SRAP Genetic Diversity Analysis of Germplasm Resources of Towel Gourd Based on SRAP Markers 1 1 1 1 2 1 CUI Jun-jie ,SONGJian-wen ,WANG Guo-ping ,LIN ming-bao ,LI Lian-fang ,HU Kai-lin 1 ( College of Horticulture,South ChinaAgricultural University,Guangzhou510642; 2GuangzhouAcademy of Agricultural Sciences,Guangzhou510308) Abstract:SRAP was used to analyse the genetic relationships of 56 angular sponge gourds and 8 sponge gourds from different areas．60 pairsof polymorphicand stableprimerswere screenedfrom 144 primerpairs.Atotal of 1433 DNA bands were amplified,1280 of which were polymorphic.The average number of polymorphic DNA bands amplified by each primer pair was21．33 and the ratio of polymorphic bands was 88．74%.UPMGA method was used to make cluster analysis and result showed that the genetic similarity coefficient among 64 towel gourds germplasm rangedfrom0．17-0．98.Thetestedspecieswereclassifiedinto2 clustergroupswiththesimilarity coeffi- cient of0．17.Thefirst group isspongegourdandthe secondgroup i