变性梯度凝胶电泳技术在微生物分子生态学的研究中的应用.pdfVIP

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 2009 5 1 2 翟振华 李艳双 1 2 ( , 100048; , 053200) : 变性梯度凝胶电泳在微生物分子生态学研究中的应用日益广 , 已成为研究微生物多样性和动态变化的有力 工具对变性梯度凝胶电泳技术中存在的问题, 如基因片段的选择共迁移背景色和异源双链分子的形成等作了综述, 以期 为进一步更好地利用该技术进行微生态的研究奠定基础 : 变性梯度凝胶电泳 微生物分子生态学 共迁移 异源双链分子 Application and Analysis of D enaturing G radient G el E lectrophoresis in M icrobialM olecular E cology 1 2 Zhai Zhenhua LiYanshuang 1 2 ( College of Lfie Science, Cap italNormal University, B eij ing 100048; Jizhou Vacational echnique Centre, Jizhou 053200) A bstrac:t Denaturant grad ient gel electro hores is ( DGGE) has beenw idely used for studies ofm icrobial divers ity and comm un ity changes in m icrob ial molecu lar ecologyThe otentials and lmi itations of the techn ique, such as chose of DNA fragm ent, com igration, background and form ation of heterodu lexeswere d iscussed It would be hel fu l to mi rove its a lication on m icroecology research K ey w ords: DGGE M icrob ial ecology Com igration H eterodu lexes ( single strand conformation olymor hism, SSCP) , ( denaturing gradient gel electro hore , sis, DGGE)( hos holi id fatty acid, PLFA)( fluorescence in situ hybrid , ization, FISH )( ter , minal restriction fragment length olymor hism, TRFLP) , , 16S rDNA [ 3]