柴油降解菌Acinetobactersp.AK5的筛选及降解性能的研究.pdfVIP

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN 年第 期 2014 8 柴油降解菌 sp. AK5 的筛选及 Acinetobacter 其降解性能研究 徐晓宇  陈敬华 （江南大学医药学院，无锡 214122） 摘 要 ： 从污水处理厂的活性污泥中分离到一株柴油降解菌，通过生理生化鉴定和16S rDNA 序列分析，鉴定该菌为不动杆 菌 Acinetobacter sp. AK5。检测了不同pH 值、NaCl 浓度、培养时间和各种柴油浓度下Acinertobacter sp. AK5 的柴油降解情况。结果表明， 该菌的最适生长初始pH 值为5-9，适合NaCl 浓度为3%-4%， 柴油浓度为5 g/L 时，该菌7 d 柴油降解率可达99%，柴油浓度为 20 g/L 时，7 d 柴油降解率也可达67%。AK5 在人工海水培养基中及无机盐培养基中生长状态良好，在海水和淡水石油污染的生物 修复中具有很好的应用前景。 关键词 ： 柴油 降解 不动杆菌 降解率 Isolation of Diesel Degrading Strain Acinetobacter sp. AK5 and Its Degrading Performance Xu Xiaoyu Chen Jinghua （ ， ， 214122 ） School of Pharmaceutical Science Jiangnan University Wuxi Abstract:  A diesel degradable bacterial strain was isolated from activated sludge and identified as Acinetobacter sp. AK5 through physiological, biochemical identification and 16S rDNA sequence analysis. Experiments of the different pH values, NaCl concentrations, culture time and diesel concentrations were detected to evaluate the diesel degradability by Acinetobacter sp. AK5. The results show that the optimal initial pH scope for the bacterial growth is from 5 to 9, the optimum NaCl concentrations is between 3% and 4%. When the diesel concentration is 5 g/L, the 7 d diesel degradation rate can reach 99%, while when the concentration of diesel is 20 g/L, 7 d diesel degradation rate can be 67%. The Acinetobacter sp. AK5 can grow well in artificial seawate