乌腺金丝桃愈伤组织中总黄酮及其金丝桃素含量测定.pdfVIP

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN ( ) 2015, 31 1 :98-103 乌腺金丝桃愈伤组织中总黄酮及金丝桃素含量测定 1，2 2 1 1 刘晓丹   张克勤  刘连  李文昌  （1. 吉林农业科技学院生物工程学院，吉林 132101 ；2. 长白山动植物资源利用与保护吉林省高校重点实验室，吉林 132101） 摘 要 ： 以乌腺金丝桃新鲜叶片为材料，建立稳定的乌腺金丝桃愈伤组织的培养体系，进一步对愈伤组织总黄酮与金丝桃 素的含量进行测定。以MS 为基础培养基，考察不同激素组合对愈伤组织诱导的影响。结果表明，乌腺金丝桃诱导愈伤组织的最 佳培养基为MS + 2，4-D 4.0 mg/L + 6-BA 0.2 mg/L ；采用超声波法提取乌腺金丝桃愈伤组织中的总黄酮和金丝桃素，分别采用紫外分 光光度法和HPLC 法测定乌腺金丝桃愈伤组织中总黄酮及金丝桃素的含量，结果表明，干燥乌腺金丝桃愈伤组织中总黄酮含量为 27.99 mg/g，金丝桃素的含量为 0.515 mg/g。 关键词 ： 乌腺金丝桃 ；愈伤组织 ；诱导 ；总黄酮；金丝桃素 DOI ：10.13560/ki.biotech.bull.1985.2015.01.015 Determination the Content of Total Flavonoids and Hypericin in Callus from Hypericum attenuatum Choisy 1，2 2 1 1 Liu Xiaodan Zhang Keqin Liu Lian Li Wenchang （1. School of Biological Engineering ，Jilin Agricultural Science and Technology College ，Jilin 132101 ；2. Key Labratory of Changbai Mountain Animal and Plant Rescources ’ Utilization and Protection of Universities in Jilin Province ，Jilin 132101 ） Abstract: With fresh leaves as test material, this study established a relatively stable system of callus culture for Hypericum attenuatum Choisy, and determined the content of total flavonoids and hypericin in callus. With MS as the basic culture medium, the effects of different hormone combinations were investigated on callus induction. The results showed that the optimum culture media for callus induction was MS + 2，4-D 4.0 mg/L + 6-BA 0.2 mg/L. The total flavonoids and hypericin of callus were extracted using ultrasonic method, respectively, using UV spect