模拟微重力对骨髓间充质干细胞全细胞瘤苗干预A549移植瘤增殖的研究.docVIP

模拟微重力对骨髓间充质干细胞全细胞瘤苗干预A549移植瘤增殖的研究 　　[摘要] 目的 比较骨髓间充质干细胞（MSCs）于不同重力环境下获得全细胞抗原（WCAs）对于人肺腺癌细胞株A549荷瘤的影响。 方法 全骨髓贴壁法原代培养小鼠MSCs，采用2D回转模式，以30 r/min水平回转模拟微重力（MMG）培养条件，并以正常重力（NG）为对照培养MSCs；随后以15 Gy的X线灭活MSCs获取WCAs，将BALB/c小鼠随机分成4组，实验组（MMG组）皮下接种WCAs为（1次/3 d，共2周）；对照组分别为PBS组，NG组，A549组；各组均采用A549细胞系皮下移植荷瘤，观察4组小鼠肿瘤生长情况；测量肿瘤直径、计算肿瘤体积，并采用免疫组化法检测增殖细胞核抗原（PCNA）表达。 结果 肺腺癌A549细胞皮下移植使小鼠成功荷瘤，MMG可促进WCAs抑制移植瘤的生长。MMG组肿瘤体积显著小于PBS组与NG组（P 中国论文网 /6/view-7215455.htm 　　[关键词] 骨髓间充质干细胞；肿瘤；模拟微重力 　　[中图分类号] R734.2 [文献标识码] A [文章编号] 1673-7210（2016）01（c）-0008-05 　　Study of simulated microgravity affecting antitumor response of mesenchymal stem cells against A549 transplantation tumor 　　LI Jing1 CHEN Jun2 LI Xiuyu1 NIU Lufang1 　　1.Department of Traditional Chinese Medicine， PLA Navy General Hospital， Beijing 100048， China； 2.Department of Acupuncture and Massage， Shaanxi University of Chinese Medicine， Shaanxi Province， Xianyang 712046， China 　　[Abstract] Objective To estimate the whole cell antigens （WCAs） of mesenchymal stem cells （MSCs） cultured in different gravity can generate different immune response against cancer in mice which induced by lung cancer cell line A549. Methods The MSCs was isolated and cultured adherent cells from marrow and a 2-dimensional clinostat （2D clinostat） was used to keep cells in continuous 2D rotation at a speed of 30 r/min around the horizontal axis， to mimc the microgravity （MMG）， and the normal gravity （NG） was used as control. And then MSCs was to irradiate to X-ray （15 Gy） to get the WCAs. The experiment group （MMG group） was immunized with WACs （once/3 d， 2 weeks） and then the mice were challenged with A549 cells； the control mice immunized subcutaneously with PBS control （PBS group）， MSCs-NG control （NG group） and A549 control （A549 group）， mice were monitored for their tumor growth by measure the diameter； then the weight and volume was plotted； the expression of PCNA was monitored by immunohistochemisty. Results A well-established lung cancer （A549） model was esta