响应面法优化川楝子总黄酮的提取工艺.docVIP

响应面法优化川楝子总黄酮的提取工艺 　　摘要：目的 优化川楝子总黄酮提取工艺。方法 以总黄酮提取率为响应值，考察料液比、提取时间、乙醇体积分数3个因素对川楝子总黄酮提取率的影响。在单因素试验的基础上，采用响应面法中的Box-Behnken模式对川楝子总黄酮的提取工艺参数进行优化分析。结果 川楝子总黄酮提取的最优工艺条件为料液比1∶35、提取时间3.0 h、乙醇体积分数70%。在此条件下总黄酮的提取率为0.587 3%。结论 实测值与响应面法回归模型高度拟合，使用该方法能合理优化川楝子总黄酮的提取工艺。 中国论文网 /6/view-7254784.htm 　　关键词：川楝子；总黄酮；响应面法；提取工艺 　　DOI：10.3969/j.issn.1005-5304.2016.04.020 　　中图分类号：R284.2 文献标识码：A 文章编号：1005-5304（2016）04-0076-04 　　Optimization of Extraction Process of Flavonoids from Toosendan Fructus by Response Surface Methodology TIAN Yong-zhi， WANG Ya-li， SUN Ru， ZHAO Jing， LIU Qun， WANG Chen-xiao， MA Yan， CAI Mei-chao （College of Pharmacy， Shandong University of Traditional Chinese Medicine， Jinan 250355， China） 　　Abstract： Objective To optimize extraction process of flavonoids from Toosendan Fructus. Methods Three parameters that significantly affect the extraction rate of flavonoids， ratio of material to water， extraction time and ethanol concentration， were taken into consideration for extraction ratio of flavonoids from Toosendan Fructus. Based on the results of single-factor experiment， the extraction parameters of flavonoids from Toosendan Fructus were optimized by Box-Behnken of response surface methodology （RSM）. Results The optimum extraction conditions were as follow： material to water ratio was 1：35； extraction time should be 3 h； ethanol concentration was 70%. The extraction ratio was up to 0.587 3% in the optimum extraction conditions. Conclusion The actual value is highly matching the theoretically value of RSM model. The results indicated that this method can rationally optimize extraction process of flavonoids from Toosendan Fructus. 　　Key words： Toosendan Fructus； flavonoids； response surface methodology； extraction process 　　川楝子为楝科植物川楝Melia toosendan Sieb. et Zucc的干燥果实，具有舒肝、行气止痛、驱虫作用，常用于治疗胸胁、脘腹胀痛、疝痛、虫积腹痛等症[1]。主要产于四川、贵州、陕西、湖北、湖南等地。有报道表明，川楝子含有黄酮类化合物[2]，而天然植物中的黄酮类化合物具有极为广泛的生理活性。本试验采用响应面法优化川楝子总黄酮的提取工艺，为进一步开发川楝子药用资源提供参考。 　　1 仪器与试药 　　川楝子（购自四川，干燥，粉碎，过40目筛，备用），经马艳老师鉴定为楝科植物川