淋巴管内皮透明质酸受体1对肺癌转移及预后评价的意义研究讲义.docxVIP

淋巴管内皮透明质酸受体1对肺癌转移及预后评价的意义研究 摘要目的检测肺癌患者血清淋巴管内皮透明质酸受体1（LYVE-1）水平，并探讨血清LYVE-1用于判断肺癌患者是否发生淋巴结转移及预测患者预后的临床意义。方法采用酶联免疫法(ELISA)检测57例肺癌患者血清中LYVE-1的水平，同时分析血清LYVE-1水平与患者临床病理特征的关系，通过受试者工作曲线（ROC）分析血清LYVE-1用于判断肺癌患者是否发生淋巴结转移的可行性，Kaplan-Meier法进行生存分析，评价LYVE-1用于预测病人预后的临床意义。结果肺癌患者血清LYVE-1为1625.0±343.0pg/mL；血清LYVE-1与肺癌患者性别、肿瘤组织学分型、血清CEA和CA125无统计学差异（P＞0.05），而与TNM分期、淋巴结转移和远处转移情况有关（P＜0.05）；血清LYVE-1用于诊断肺癌患者发生淋巴结转移的曲线下面积为0.728（95% CI：0.685-0.747，P＜0.05）；LYVE-1的为1821pg/mL时，其诊断的敏感性为79%，特异性为68%；血清LYVE-1大于1821pg/mL的肺癌患者预后要比血清LYVE-1小于1821pg/mL的肺癌患者预后差，两组总生存时间有统计学差异（P=0.035）。结论血清LYVE-1可用于确定肺癌患者是否发生淋巴结转移，并可用于评估病人预后。 关键词：淋巴管内皮透明质酸受体1；肺癌；转移；预后 The significance of serum lymphatic vessel endothelial hyaluronan receptor-1in the metastasisand prognosis of lung cancer AbstractObjective Todetect the level of the serum lymphatic vessel endothelial hyaluronan receptor-1(LYVE-1) in lung cancer patients, and to investigate the significance of serum LYVE-1 in the metastasis and prognosis of lung cancer. Methods: Serum levels of LYVE-1 in 57 lung cancer patients were detected by Enzyme-linked immunosorbent assay(ELISA); Clinical and pathologic features were analysed with levels of LYVE-1; The specificity and sensitivity of serum LYVE-1in determine whether lymph node metastasis or not were analyzed by receiver operating characteristic(ROC) curve; Kaplan–Meier methodwas used to evaluate overall survival according tocutoff serum LYVE-1 level in patients.Results: Serum LYVE-1 levels were 1625.0±343.0pg/mL; A high cancer staging, occurrence of lymph-node metastases, and occurrence of distant metastases patient wereassociated with high LYVE-1 levels; A LYVE-1 value = 3542pg/ml had a sensitivity of 68% and a specificity of 79%, with an area under the curve (AUC) of 0.728, p0.05, for identifying lymph node metastases in patients with lung cancer, and the higher serum LYVE-1 level predicates poorer prognosis. Conclusion: The serum levels of LYVE-1 might be a helpful biomarker in identified lymph node metastases, it a