Bioinf2资料.ppt

The GenBank?release notes?for release 162.0 (October 2007) state that from 1982 to the present, the number of bases in GenBank has doubled approximately every 18 months. * * * HTG和HTC序列完整后转移到相应的organismal divisions * * GenBank的主要目标是“bank of gene”，一般所包含的序列有比较好的注释（如基因区等） EST、GSS不需要提供注释，往往也未包含完整基因，所以单独成库。 * Transcript-Based Build Procedure /UniGene/help.cgi?item=build1 Genome-Based UniGene Build Procedure /UniGene/help.cgi?item=build2 * TSA指由多条EST拼接而成，或转录组测序序列拼接而成（即序列来源于多个独立“clone”）。 如果是一个clone经多次测序获得不同片段，再拼接而成，则属于传统的单条序列（如：单条EST/cDNA）。 * 例子中序列由多条原始序列拼接得到 * * * * * * * “the team(s) able to sequence 100 human genomes within 30 days to an accuracy of 1 error per 1,000,000 bases, with 98% completeness, identification of insertions, deletions and rearrangements, and a complete haplotype, at an audited total cost of $1,000 per genome.” ?Kedes, L.; Campany, G. (2011). The new date, new format, new goals and new sponsor of the Archon Genomics X PRIZE Competition.?Nature Genetics?43?(11): 1055–1058.?doi:10.1038/ng.988.?PMIDIn August 2013 the Archon Genomics X PRIZE was cancelled, as the founders felt it had been Outpaced by Innovation, and was not incentivizing the technological changes?[27] In January 2014, Illumina launched its HiSeq X Ten Sequencer which delivers the first $1000 genome at 30x coverage,[28]?including reagent costs ($797), instrument depreciation ($137 per genome), and sample preparation ($55-$65 per genome).?[29]  * Statistics: /genomes/static/gpstat.html * 可以查询已经完成基因组测序信息（物种、基因组大小、注释基因数目、染色体、GC%、序列数据、完成测序单位、文献等信息） * /wiki/content/bioproject%E5%92%8Cbiosamle-%EF%BC%9A%E4%BF%83%E8%BF%9B%E6%8D%95%E8%8E%B7%E5%92%8C%E7%BB%84%E7%BB%87%E5%85%83%E6%95%B0%E6%8D%AE%E7%9A%84ncbi%E6%95%B0%E6%8D%AE%E5%BA%93 * Submit variations 50 nucleotides in length to the Database of Genomic?Structural Variation (/dbvar) * * dbVar is a database of genomic structural variation that allows you to search, view, and download variant data from studies submitted for