3茶叶农药残留与重金属检测技术PesticideResiduesandHeavyMetalTestTechnologyofTea汇总.pptVIP

10 凝胶渗透色谱(GPC)10. Gel permeation chromatography 凝胶渗透色谱技术，已成为农药多残留分析中的通用净化方法。其作用类似一组分子筛，将样品溶液加到柱子上后，用溶剂淋洗，分子量大于农药的类脂肪、色素、蜡质等先被淋洗出来，然后农药按分子量大小相继被淋洗出来。 A type of?size exclusion chromatography?(SEC), that separates?analytes?on the basis of size. The technique is often used for the analysis of?polymers. It’s like a set of molecular sieve, when liquid sample add in column, then elute with solvent, larger molecular weight fat, pigment, waxiness etc. are first eluted, then pesticides are eluted by size of molecular weight. 常用的淋洗剂有环己烷/二氯甲烷、甲苯/乙酸乙酯、二氯甲烷/丙酮等。 Common elution are cyclohexane、dichloromethane, methylbenzene/ethyl acetate, dichloromethane/acetone, etc. 当被分析的试样随着淋洗溶剂引入柱子后，溶质分子即向填料内部空洞扩散，较小的分子除了能进入大的孔外，还能进入较小的孔；较大分子则只能进入较大的孔；而比最大的孔还要大的分子就只能留在填料颗粒之间的空隙中。因此，随着溶剂的淋洗，大小不同的分子就得到分离，较大的分子先被淋洗出来，较小的分子较晚被淋洗出来。溶质分子的体积越小，其淋洗出体积越大。其淋出体积仅仅由溶质分子尺寸和载体的孔尺寸决定，分离完全是由于体积排除效应所致，故称为体积排除机理。 Separation occurs via the use of porous beads packed in a column. The smaller analytes can enter the pores more easily and therefore spend more time in these pores, increasing their retention time. Conversely, larger analytes spend little if any time in the pores and are eluted quickly. All columns have a range of molecular weights that can be separated. The smaller the solvent molecular sizes are, the larger elution sizes are. Elution volume is only determined by solvent molecular dimension and pore size, the separation is only resulted from size exclusion, so it’s called size exclusion mechanism. 11 基质固相分散技术(MSPD)11. Matrix solid-phase dispersion 其原理是将涂渍有C18 等多种聚合物的单体固相萃取材料与样品一起研磨，得到半干状态的混合物并将其作为填料装柱，然后用不同的溶剂淋洗柱子，将各种待测物洗脱下来。其优点是浓缩了传统的样品前处理中的样品匀化、组织细胞裂解、提取、净化等过程，不需要进行组织匀浆、沉淀、离心、pH调节和样品转移等操作步骤，避免了样品的损失。 grind sample with monomer solid phase extraction material which coating with C18 and other polymers, then get semi-dry mixture, put the mixture as fillings into column and elute with different solvents and waiting for the elution of determinand. it extracts processes of traditional sample preparatio