告达亭通过Wntβcatenin信号通路影响胃癌细胞系增殖.pdf

的表达均逐渐下降，而1et．7amiRNA的表达逐渐上升。 本论文研究结果表明，告达亭可以抑制胃癌细胞株(AGS和 HGC．27)的增殖并诱导其凋亡，其诱导凋亡的原因是通过影响 新机制，为临床治疗胃癌提供了一种新的思路。 信号通路 ABSTRACT A10tof haveindicatedthatcaudatincouldinhibitcells papers andinducecells as proliferation apoptosis，suchgastic carcinomaand wasconsideredthatcaudatin cancer,which lung possesses thecharacteristicsof ithas thatcaudatin anti—tumor．Although reported couldinhibit cells andinducecells gastricproliferation apoptosis，few haverevealedthe mechanismsofcaudatinin studies underlying gastic cells． In influenceof on our detectedthe caudatin presentstudy,we gastric cells(AGSandHGC一27)in MTT also proliferationbyusing assay．we theeffectofcaudatinon cells(AGSandHGC·27)m analyed gastric themethodsofHoechst33258 andFACS apoptosisthrough staining blot demonstratedthat assays．Inaddition，Westernanalysis Caspase-9， andPARPwere resultedincell activated，which Caspase-3，Caspase-8 showedthatcaudatininducedG1／Sarrestand apoptosis．FACSassays the of ofthe1 levels down-regulatedexpressionCDK2，yet，theproteinp2 was ina manner．Theseresultsind