马齿苋黄酮对果蝇唾液腺染色体的影响by叶子.doc

PAGE 4 闽南师范大学 毕业论文 马齿苋黄酮对果蝇唾液腺染色体的影响 The Effects of Flavones of Purslane to Salivary Gland Chromosome of Drosophila 姓 名： 叶芳美 学 号： 101301104 系 别： 生物科学与技术学院 专 业： 生物科学 年 级： 10级 指导教师： 曹芳 2014 年 05 月 08 日  PAGE \* MERGEFORMAT I 摘要 用含有马齿苋黄酮0%、1%、2%、3%、4%和5%(体积比)的培养基培养野生型果蝇，将培养获得的三龄幼虫通过唾腺剥离、压片、染色等完整的制片过程探索马齿苋黄酮对果蝇唾液腺染色体的影响。通过对比，发现同样用改良苯酚品红溶液染色5min,培养基中含有马齿苋黄酮的唾腺染色体臂较伸展，带纹较清晰，说明马齿苋黄酮对果蝇唾腺染色体有影响。 关键词：马齿苋；黄酮；果蝇；唾液腺染色体 Abstract The wild Drosophila cultivated in the medium Containing flavonoids were 0%, 1%, 2%, 3%, 4% and 5%( volume ratio), making rule of temporary chromosome specimen was explored the flavonoids from Portulaca oleracea effects on the salivary gland chromosome of Drosophila throughout the complete course. By comparison, the staining effect of modified phenol fuchsine for 5min, Salivary gland chromosome arms in medium containing purslane flavone is stretching bands were clear, this shows that flavonoids from Portulaca oleracea have effects on salivary gland chromosome of Drosophila. Keyword: Portulaca oleracea ; Flavonoid; Drosophila; Salivary gland chromosome   PAGE \* MERGEFORMAT I 目 录  TOC \o 1-4 \h \z \u  HYPERLINK \l _Toc386915455 摘要  PAGEREF _Toc386915455 \h I  HYPERLINK \l _Toc386915456 Abstract  PAGEREF _Toc386915456 \h I  HYPERLINK \l _Toc386915457 目 录  PAGEREF _Toc386915457 \h I  HYPERLINK \l _Toc386915458 前 言  PAGEREF _Toc386915458 \h 1  HYPERLINK \l _Toc386915459 1材料与方法  PAGEREF _Toc386915459 \h 1  HYPERLINK \l _Toc386915460 1.1材料  PAGEREF _Toc386915460 \h 1  HYPERLINK \l _Toc386915461 1.1.1动植物材料  PAGEREF _Toc386915461 \h 1  HYPERLINK \l _Toc386915462 1.1.2药品及试剂  PAGEREF _Toc386915462 \h 1  HYPERLINK \l _Toc386915463 1.1.3仪器  PAGEREF _Toc386915463 \h 1  HYPERLINK \l _Toc386915464 1.2实验方法  PAGEREF _Toc386915464 \h 2  HYPERLINK \l _Toc386915465 1.2.1马齿苋黄酮的提取