LAMP检测-戴婷婷剖析.docVIP

环介导等温扩增技术的应用研究进展 戴婷婷1，陆辰晨2，郑小波2 （1南方现代林业协同创新中心，南京林业大学林学院，江苏南京210037； 2南京农业大学植物保护学院，江苏南京210095；） 摘要：环介导等温扩增技术（Loop–mediated isothermal amplification，LAMP）采用特异识别靶序列上6个位点的4条引物及一种具有链置换活性的DNA聚合酶，在等温条件（60℃~65℃）下60 min左右进行核酸扩增，其扩增效率可达到109~1010个数量级，具有特异性强、灵敏度高、等温、操作简单和检测结果快速等优点，已广泛应用于病毒、细菌、寄生虫、菌物等病原物的检测中。本文对LAMP技术原理及其在病原物检测中的应用做了简要综述。 关键词： 环介导等温扩增技术；应用；检测；病原物 中图分类号： 文献标志码： 文章编号： Application Research Progress Of Loop-Mediated Isothermal Amplification DAI Ting-ting1; LU Chen-chen2; ZHENG Xiao-bo2 (1Southern China Collaborative Innovation Center of Sustainable Forestry, College of Forestry, Nanjing Forestry University, Nanjing, Jiangsu 210037, China；2College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China) Abstract：A new method of gene amplification called Loop-mediated isothermal amplification (LAMP) has been developed in recent years. LAMP assay possess characteristic of simpleness, fleetness and high specificity. The whole procedure is very simple and rapid which can be completed in less than 80 min under isothermal conditions employing a set of six specially designed primers of a target gene, by incubating all the reagents in a single tube. LAMP does not require a thermal cycler and can be performed simply with a heating block or water bath. Considering the advantags of rapid ampli?cation, simple operation and easy detection, LAMP has potential applications for diagnosis as well as surveillance of infectious diseases in developing countries without requiring sophisticated equipment or skilled personnel. LAMP has been widely applied to the detection of such pathogens as virus，bacteria, parasite, fungi etc. This article summarized the principle of LAMP，and the application in detection of pathogenic microorganisms. KEY WORDS：Loop-mediated isothermal amplification; application; detection; pathogenic microorganisms 核酸扩增技术是分子生物学领域的一项重要的研究手段，核酸扩增技术主要包括常规PCR 为基础的变温扩增技术以及等温扩增技术两大类。目前，变温扩增技术主要包括普通PCR、巢式PCR、多重PCR、实时荧光定量PCR等，这些技术在病原微生物的检测中发挥着重要的