制何首乌多糖提取纯化及抗衰老作用地研究论文.docVIP

制何首乌多糖的提取纯化及抗衰老作用研究 第三部分： 用制何首乌多糖PRPMA、PRPMB、PRPMC生理盐水溶液对D-半乳糖衰老模型小 鼠灌胃。结果表明: 制何首乌多糖能较显著提高小鼠血清及脑组织中SOD活性， 降低MDA含量，此外还能显著提高正常小鼠GSH－PX血清及脑组织中的活性，同时 证明了其中PRPMB为主要活性部位。 关键词：制何首乌；多糖；提取工艺；纯化工艺；抗衰老 -2- 英文摘要 Study on the extraction、purification and aging- resistant of the polysaccharide from Radix Polygoni Multiflori Preparata Wang Ting （Science of Pharmaceutical Analysis） Directed by Liang Shengwang Abstract The dry root of polygonum multiflorum thunb.(polygonaceae) has flat qualities, a little bitter and flavor. It is good at disposing hotness and poison, subsiding swelling and bump .when it has been prepared it becomes warmer and sweeter, can benefit liver and kidney, nourish the blood, make hair darker, and strengthen the bones and muscles. The prepared polygonum multiflorum is used more often than unprepared. In the paper, the prepared polygonum multiflorum was used as research material and its water- -soluble polysaccharide was explored in the following respects, including extraction, content determination, purification, fraction and pharmacological study, the results were as follows: The first part: the polysaccharides from Radix Polygoni Multiflori Preparata (PRPM) are extracted by the methods of wate extracting. Factors that had influence on the yield of PRPM were mainly considered, including extraction temperature, extraction time, extraction times, the solvent-solid ratio,etc. On the basis of single factor experiments, orthogonal design was adopted to search the optimum extracting parameter of PRPM.. The result showed that optimum extracting parameter as follows: 90℃ of extraction temperature, 1h of extraction time, 2 times of extraction times， 15:1 of the solvent-solid ratio and pH7.0 were suitable. Taking glucose as control, the phenol-sulfuric acid method examining content of PRPM was established. The results showed that the revised method was stable and had good repeatability and