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α-细辛脑对青霉素诱导大鼠癫痫发作的实验研究 任亮1，常陆林1，马菲2，杜钢军3 （1．漯河医学高等专科学校，河南 漯河 462002；2.漯河市源汇区妇幼保健院，河南 漯河 462002；3.河南大学药学院，河南 开封 475001） [摘要] 目的：观察α-细辛脑对青霉素诱导大鼠癫痫急性发作的作用并分析其抗癫痫的作用机制。 方法：将istar大鼠随机分成对照组、模型和组对照组和模型大鼠腹腔注射生理盐水0万U/kg）诱导大鼠。观察并比较3组大鼠的行为变化1β ( IL-1β)与肿瘤坏死因子(TNF-α)含量。 结果：α-细辛脑可使癫痫大鼠发作程度减轻和发作潜伏期延长；使癫痫大鼠脑内MDA 、TNF-α和IL-1β含量明显降低，SOD活性明显升高。 结论：α-细辛脑可有效地防治癫痫的发作并有效地改善癫痫大鼠的预后情况。其抗癫痫作用与调节脑组织的相关因子，提高其脑组织清除自由基，增强抗氧化作用有着密切关系。 [关键词] α-细辛脑；癫痫模型；青霉素 [中图分类号] R965 [文献标示码]A Experimental Effect of Alpha-asarone on Epilepsy Induced by Penicillin in Rats REN Liang1,CHANG Lu-lin1 ,MA Fei2,DU Gang-jun3(1.Luohe medical college,Henan Luohe 462002,China;2.The Women and Children Health Hospital of Yuanhui District in Luohe ,Luohe 462002,Henan China;3.Pharmaceutic college of Henan university,Henan Kaifeng 475001,China) ABSTRACT: OBJECTIVE To observe the effects of alpha-asarone on epileptic be- havior induced by penicillin in? rats and analysis it’s mechanism. METHODS Wistar rats were randomly divided into normal control group, epileptic model group and treated group.The normal control group and the epileptic model group were treated with normal saline,the treated group was treated with alpha-asarone,after 20 minutes epilepsy was induced with penicillin in both of the epileptic model group and the treated group. The changes in epileptic behavior among three groups were compared, the activity of SOD 、MDA、IL-1β and TNF-αin rat brain was investigate. RESULTS Compared with epileptic model group,the treated group showed a prolonged latent period and reduced times of epileptic behavior, Alpha-asarone could promote the activity of SOD and decrease IL-1β、 TNF-αand MDA in rat brain. CONCLUSION Alpha-asarone has anti-epileptic effect, which might be related to its anti-oxidanttion． KEY WORDS: Alpha-asarone;Epilepsy Model;Penicillin α-细辛脑（Alpha-asarone）又名α-细辛醚，是中药石菖蒲的主要有效成分，具有止咳、平喘、祛痰、镇静、抗惊厥等药理作用。有研究表明，α-细辛。Wistar大鼠，雌雄兼用，180～220 g，由河南省医学实验动物中心提供。青霉素注射液，华北制药股份有限公司IL-1β放射免疫检测试剂盒，华美生物工程公司。