血卟啉衍生物光动力作用杀伤人胰腺癌细胞的实验研究(精选).docVIP

血卟啉衍生物光动力作用杀伤人胰腺癌细胞的实验研究作者：赵玉沛　杨波　张太平　蔡力行　朱预单位：100730 中国医学科学院 中国协和医科大学 北京协和医院外科关键词：血卟啉衍生物；光化学疗法；胰腺肿瘤摘　要：目的　探讨光动力作用(PDT)对体外培养的人胰腺癌细胞的杀伤效应。　方法　以2株人胰腺癌细胞系P3和SW1990为研究对象，采用血卟啉衍生物(HPD)作为光敏剂，用高压钠灯（功率密度25 mW/cm2)为光源，以系列浓度的HPD经不同剂量的光照后，用MTT法测定PDT对胰腺癌细胞的相对抑制率。　结果　随光敏剂浓度的升高和照光剂量的增加，光动力作用对细胞的相对抑制率逐渐增大，在低光动力剂量下明显上升，随后上升渐趋缓慢达平台期；相同光动力剂量下，PDT对2株细胞的相对抑制率存在明显差异(P＜0.01)，LD90剂量条件亦不同。　结论　光动力作用对体外培养的人胰腺癌细胞具有明确的杀伤效应，其对细胞的相对抑制率具有显著的剂量效应关系；2株人胰腺癌细胞对PDT的敏感性不同。 Abstract：Objective　To observe the effect of photodynamic therapy(PDT)on human pancreatic carcinoma cell lines in vitro.　Methods　Two lines of human pancreatic carcinoma cell P3 and SW1990 were studied to investigate the killing role of PDT with hematoporphyrin derivative(HPD) as photosensitizer and sodium lamp as light source. The cultured cells were treated with serial concentrations of HPD followed by irradiation of different dosages of visible light, then MTT colorometric assay was applied to measure the relative inhibitory rate of PDT for the cells.　Results　The relative inhibitory rate of PDT for the cells elevated along with the increase in the concentration of sensitizer and dose of light. Under the low photodynamic dose, it increased rapidly, and gradually slowed down to reach the plateau. Under the same photodynamic dose, significant difference in the inhibitory rate between the two lines was observed (P＜0.01), and the LD90 was also different. Conclusions　PDT has a significant killing effect on human pancreatic carcinoma cell lines in vitro, and its relative inhibitory rate appears to be correlated with the dose of sensitizer and light irradiation. The sensitivity of the two cell lines to PDT is different. Key words：Hematopor phyrin derivative；　Photochemotherapy；　Pancreatic neoplasms 　　由于胰腺癌早期缺少特异性症状， 发现比较晚， 现有治疗方法如手术、化疗和放疗等疗效均不佳。国外自80年代初开始探索将光动力治疗用于胰腺癌，并进行了一些细胞和动物实验研究。我们试图在细胞水平探讨光动力治疗对体外培养的人胰腺癌细胞的作用。 　　材料与方法 　　一、 材料 　　1．光敏剂：血卟啉衍生物(HPD)针剂:100 mg/20ml，主要吸收峰在320～500 nm，避光保存于4 冰箱。 　　2．光源：高压钠灯：波长589 nm，总功率400 W，照射实验所用的功率密度为25 mW/