四川省主要药用植物病害种类调查和川麦冬炭疽病的研究.pdfVIP

侵染来源之一。 4．五种杀菌剂对川麦冬炭疽菌的杀菌作用测定 选择了五种常用的杀菌剂：退菌特、甲基托布津、百菌清、多菌灵和代森锰锌， 采用含药平板法和玻片上孢子萌发法，测定了它们对二种炭疽病菌菌丝生长和孢子萌 发的抑制作用，并测定了抑菌中量(EC50)。结果是：五种杀菌剂对川麦冬炭疽病菌 菌丝生长和孢予萌发均有抑制作用，多菌灵对病菌菌丝生K的抑制作用最强，但对孢 子萌发的抑制作川稍差；代森锰锌对病菌菌丝生长的抑制作用最弱，但对c liliacearum孢子萌发的扣】制作用最强；退菌特对病菌菌丝生长的抑制作用较强，对 C gloeosporioides孢子萌发的抑制作用最强：甲基托靠津和百菌清对病菌菌丝生长和 孢予萌发均有较强的抑制作用。 关键词：四川；药用植物；病原鉴定：麦冬炭疽病；生物学特性 Studies on Diseases Species of Major Medicinal Plants in Sichuan and Anthracnose(Colletotrichum spp．) of Ophiopogon japonicus Chen Xiao-hong (Sichuan Agricultural Universi吼Ya’an 625014，Sichuan，P．R．China) Abstract ，This paper reposed lhe investigation results on diseases species of major medicinal plants in Sichuan and anttuacnos：：of Ophiopogonjaponicus． i．The disease species of major medicinal plants in Sichuan In 2003～2004，we made a field survey of the major medicinal plants diseases in Sichuan province．The diseased samples were collected and isolated the pathogens tO identify the species of pathogens．One hundred and thirty—six species of diseases were found in thirty-eight species ofmajor medicinal plants in Sichuan．Among them twenty—six species of diseases were new domestic records．According to the investigation results，a list of diseases in these medicinal plants was made． 2 The investigation on anthraenose of Ophiopogonjaponicus The samples of anthracnose of Ophiopogon japonicus were collected from Santai， Mianyang and Ya’an．The fungi caused the disease were isolated from smnples and identified for the species of colletotricum spp．．The results showing that the anthrocnose of Ophiopogon japonicus was caused by two species of colletotricum．They are Colletotrichum liliacearum(West，)Duke．and Colletotrichum gloeosporioides(Penz．) Penz．et Saccado．The biological characteristics of the two pathogens were investigated by agar plate and glass slide methods．The results indicated that there were some differences in biological characteristics(the effect of temperature，humidity,pH value，illumination and nutrition on mycelial growt