自然杀伤T细胞在髋关节骨关节炎的表达与临床意义.docVIP

自然杀伤T细胞在髋关节骨关节炎的表达与临床意义 　　[摘要] 目的 探讨自然杀伤T细胞（NKT）在髋关节骨关节炎（HO）患者的表达与临床意义。 方法 收集45例HO患者外周血，采用流式细胞术检测其内NKT及其胞内细胞因子γ干扰素（IFN-γ）及白介素4（IL-4）的含量。并与45例类风湿性关节炎（RA）患者进行对比。结果 与健康对照组比较，RA及HO组患者NKT及IFN-γ均显著降低差异有统计学意义（P0.05）；在IL-4的比较方面，RA组显著低于对照组，但HO组IL-4与对照组比较，差异无统计学意义（P=0.098）。治疗后RA及HO组NKT、IFN-γ及IL-4均较治疗前不同程度上升，均以HO回升幅度明显，差异有统计学意义（P0.05），但治疗后RA组IFN-γ频率较前无统计学变化，而HO组较治疗前明显上升，且HO组IFN-γ频率明显高于RA组（P=0.006）。结论 NKT在HO免疫机制当中起到重要作用，其细胞因子IL-10在HO与RA的鉴别起到重要作用，且IFN-γ与疗效密切相关。 　　[关键词] 骨关节炎；自然杀伤T细胞；细胞因子；γ干扰素 　　[中图分类号] R684.3 [文献标识码] A [文章编号] 1674-0742（2014）06（b）-0025-03 　　[Abstract] Objective To explore the expression and clinical significance of natural killer T cells （NKT） in patients with hip osteoarthritis（HO）. Methods The peripheral blood of 45 patients with HO was collected. Flow cytometry was used to detect the content of NKT and intracellular cytokine gamma interferon（IFN-γ） and interleukin 4（IL-4）， and compared with 45 patients with rheumatoid arthritis （RA）. Results Compared with the healthy controls， NKT and IFN-γ of the patients in RA group and HO group were significantly lower（P0.05）； in terms of IL-4， compared with the control group， the IL-4 of RA group was lower， but the difference in IL-4 between HO group and control group was not statistically significant（P=0.098）. After the treatment， the level of NKT， IFN-gamma and IL-4 of RA group and HO group increased at different levels as compared with those before treatment， and those of HO group increased significantly（P0.05）； IFN-gamma frequency of RA group after treatment showed no statistically change as compared with that before treatment， but that of HO group increased significantly compared with that before treatment， and IFN-gamma frequency of HO group was obviously higher than that of RA group（P=0.006）. Conclusion The NKT play an important role in the HO immunity mechanism， the cytokine IL-10 plays an important role in differentiating HO and RA， and is closely related to the curative effect of IFN-gamma. 　　[Key words]