武昌鱼中挥发性成分的HS―SPME和GC―MS分析.docVIP

武昌鱼中挥发性成分的HS―SPME和GC―MS分析 　　摘要：以湖北省特色水产武昌鱼（Megalobrama amblycephala）为材料， 　　关键词：顶空固相微萃取；气质联用仪；武昌鱼（Megalobrama amblycephala）；挥发性成分 　　中图分类号：O657.63；O657.7+1 文献标识码：A 文章编号：0439-8114（2014）04-0907-05 　　Analyzing of Volatile Components in Megalobrama amnblycephala by Headspace 　　Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry 　　LI Yang1，2，WANG Chao1，HU Jian-zhong1，LIAO Li2，WANG Jun2，WANG Lan2，WU Wen-jin2， XIONG Guang-quan2，CHEN Wei2， LI Dong-sheng1，QIAO Yu2 　　（1. College of food and pharmaceutical， Hubei University of Technology， Wuhan 430068， China； 　　2. Research Institute of Agricultural Products Processing and Nuclear-Agricultural Technology， Hubei Academy of Agricultural Sciences/Agricultural Products Processing Subcenter of Hubei Agricultural Science Technology Innovation Center， Wuhan 430064， China） 　　Abstract： The volatile components of Hubei special aquatic product， Megalobrama amblycephala were extracted by headspace solid-phase microextraction（HS-SPME） and identified by gas chromatography-mass spectrometry（GC-MS）. The extractions were optimized with reference to these high content of compound in the fish as hexanal， benzaldehyde， 1-octen-3-ol， octanal， （Z）-1， 3， 6-octatriene， 3，7-dimethyl， （E）-2-octenal， nonanal， （E）-2-nonenal， nonanol， （E， E）-2， 4-decadienal. The optimized conditions of HS-SPME were as follow： divinyl benzene-carboxen-polydimethylsiloxane（DVB-CAR-PDMS） fiber， the quality of fish （3 g）， extraction time （60 min）， extraction temperature （60 ℃）， saturated salt water （10 mL）（36% NaCl）. The extraction of volatile components from fresh Megalobrama amblycephala were determined using the optimized conditions of HS-SPME coupled with GC-MS. 62 volatile components were identified by comparing their mass spectra with those contained in the NIST08 mass spectral database and reference documents. The greatest relative amounts of aldehydes， ketones and alcohols were 21.66%， 4.84% and 54.29%， respectively. 　　Key words：