姜黄素对人结肠癌细胞HCT.docVIP

姜黄素逆转结肠癌裸鼠移植瘤多药耐药的研究 卢伟东 ，傅仲学，覃 勇 ，李 雷 ，杨 闯 （400016 重庆重庆医科大学附属第一医院普通外科HCT-8/VCR多药耐药的逆转作用及其可能机制。方法 将HCT-8/VCR种植于裸鼠皮下，建立肿瘤耐药模型。移植瘤裸鼠20只依据治疗方法的不同分为对照组（PBS）、长春新碱(Vincristine,VCRmg/kg)组、姜黄素（50 mg/kg）组、姜黄素联合VCR（50 mg/kg姜黄素+2 mg/kgVCR）4组。治疗2周后收集标本，称量各组瘤体质量，采用RT-PCR、Western blotMDR1 mRNA、Survivin mRNAP-gp、SurvivinHE染色观察瘤组织细胞形态学变化，评估姜黄素在不同处理因素中逆转肿瘤多药耐药的效果。结果 姜黄素联合VCR能显著抑制瘤体的生长，姜黄素联合VCR组瘤质量（0.42±0.23）g明显低于对照组（1.21±0.25）gVCR组（1.13±0.27）g组（0.75±0.27）gP0.05）。RT-PCR、Western blotMDR1 mRNA、Survivin mRNA和P-gp、SurvivinVCR组MDR1 mRNA（0.18±0.05）、Survivin mRNA（0.63±0.04）P-gp（0.15±0.02）、Survivin（0.31±0.09）和姜黄素组MDR1 mRNA（0.39±0.11）、Survivin mRNA±0.12）P-gp（0.34±0.07）、Survivin±0.11）MDR1 mRNA (0.99±0.23）、Survivin mRNA±0.32）P-gp（0.71± 0.23）、Survivin±0.15）P0.05）。HE病理切片显示姜黄素组和姜黄素联合VCR组较其他2组有较多的淋巴细胞浸润，核固缩较多，核分裂相少，坏死更为广泛。结论 多种机制参与姜黄素体内逆转肿瘤多药耐药，姜黄素可能通过下调MDR1 mRNA、Survivin mRNA和P-gp、Survivin抗药性, 多药移植, 异种小鼠, 裸Curcumin reverses multidrug resistance in HCT-8/VCR nude mice xenograft Lu Weidong, Fu Zhongxue, Qin Yong, Li Lei, Yang Chuang (Department of General Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China) [Abstract] Objective To investigate the multi-drug resistance reversal effects of curcumin on xenografts derived from multi-drug resistant human colon carcinoma cell line HCT-8/VCR, and to explore the possible mechanism. Methods The models of HCT-8/VCR xenograft (50 mm3) in nude mice were established through implanting 0.1 ml cells at 4?107 cells/ml. Twenty nude mice bearing HCT-8/VCR tumors were randomized into 4 groups according to different treatments, that is, group A: control group, group B: VCR group (2 mg/kg injected around the tumor mass, once per 2 d, for 7 times), group C: curcumin group(50 mg/kg, injected around the tumor mass, once per 2 d, for 7 times), group D: curcumin plus VCR group (as combined the treatment of groups B and C). Tumor volume was calculated with L×D2/2 which were measured once per 2 d, for 14 d. Tumor weight was