柑橘黄龙病株内生细菌群落结构的多样性.docVIP

柑橘黄龙病株内生细菌群落结构的多样性 刘波(，郑雪芳1，孙大光1,2，阮传清1，范国成3，段永平4 1福建省农业科学院农业生物资源研究所，福州 350003；2福建师范大学生命科学学院，福州350003； 3福建省农业科学院果树研究所福州，350003 ；4美国农业部佛罗里达园艺试验室，弗罗里达34945 摘要：采用PCC（Pearson correlation coefficient）、系统聚类和多样性指数研究了柑橘黄龙病citrus huanglongbing, HLB）植株不同部位叶片、枝条及其根部内生细菌的相互关系及其群落结构多样性。结果表明，从供试柑橘中分离得到26株内生细菌（叶片10株，枝条14株，根部2株）隶属于10个属19个种。Nested-PCR结果显示，柑橘植株不同空间叶片中，黄龙病病原的阳性检出率为58.3%。用LSD法比较显示，柑橘内生细菌在不同器官的量大小依次为根叶枝条。不同叶片和枝条内生细菌差异不显著，而不同的叶片和枝条内生细菌量差异显著柑橘内生细菌PCC分析表明，Bacillus pumilus和Bacillus sp.与柑橘黄龙病病原菌呈显著负相关Bacillus sp.和Bacillus pumilus与Bacillus subtilis呈显著正相关。Bacillus brevisEscherichia hermannii与Bacillus vesiculariss存在显著正相关。不同器官的内生细菌之间负相关。不同器官之间表明叶片与枝条0.55，16S rDNA序列聚类分析内生细分为两大类，第I大类为革兰氏阳性菌，第II大类为革兰氏阴性菌。第I大类又可以分为芽孢杆菌和两个亚类，第II大类可以分两个亚类。柑橘黄龙病病原与叶片内生细菌可以分为三类，第I类第II类与黄龙病病原菌存在负相关性第III类细菌柑橘，第Ⅰ类特点，第Ⅱ类特点，第Ⅲ类特点。 SHANNON（H1）指数叶片最高，根部最低。SIMPSON（D）指数枝条最高，根部最低。Pielou指数根部最高，枝条最低。 关键词：柑橘；黄龙病；内生细菌；多样性；群落结构 The community structure endophytic bacteria in different parts of huanglongbing-affected citrus plants LIU Bo1*, ZHENG Xue-fang1，SUN Da-guang1，2， RUAN Chuan-qing1, FAN Guo-cheng3, DUAN Yong-ping4 1. Agricultural Bioresource. Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China； 2. College Of Life Science Fujian normal university, Fuzhou 350003, China; 3. Research Institute. of Pomology , Fujian Academy of Agricultural Sciences, Fuzhou 350003, China； 4 .USDA-ARS-USHRL, Fort Pierce, FL 34945, U.S.A Abstract: The analyses methods of Pearson correlation coefficient (PCC), hierarchical cluster analysis and diversity index were used to study the relevance between citrus huanglongbing (HLB) and the endophytic bacteria in different branches and leaves as well as roots of huanglongbing (HLB-affected citrus trees. The results showed that 26 isolates (10 isolates from leaves, 14 isolates from branches and 2 isolates from roots) from HLB-affected citrus plants belonged to 19 bacterial species in 10 Genus. Among the 12 tested leaf samples, 58.3% of them were positiv