Rare allele of OsPPKL1 associated with grain length causes.pptVIP

Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice 研究意义 水稻产量 定位并克隆新的粒形基因对探索水稻产量形成调控机制、提高水稻产量及改善稻米品质具有十分重要的意义！ 研究材料 Map-Based Cloning of qGL3 初步定位：N411×N643 F2 精细定位：N411×93-11 NIL qGL3 is on the long arm of chromosome 3 between InDel markers XJ39 and XJ26. ORF4 was most likely the candidate gene for qGL3. Confirmation of OsPPKL1 as qGL3 1. qGL3 is OsPPKL1 2. OsPPKL193-11 plays an inhibitory role in regulating the grain length, OsPPKL1N411 abolishes its inhibitory role 3. The Kelch domains are essential for the OsPPKL1 Anticancer Agents Functions of Two OsPPKL1 Homologs 1. All three OsPPKLs exhibit similar expression profiles in various rice organs, but OsPPKL2 expresses at a lower level than OsPPKL1 and OsPPKL3 2. It is possible that the OsPPKL2 subgroup may also positively regulate cell elongation or division, whereas the OsPPKL1/3 subgroup plays a negative role Anticancer Agents Sequence Polymorphisms of qGL3 in Rice Germplasms The qgl3 is a rare allele in rice SNP1 is a functional mutation for long grain Biological Roles of qgl3 1. It is very likely that the long glumes of NIL-qgl3 result from an increase in cell numbers 2. The qgl3 allele has a great potential in breeding elite rice varieties Anticancer Agents qgl3 Enhances Rice Yield Significantly 1. The qgl3 allele can be applied in breeding elite varieties to improve rice grain production without sacrificing grain quality. 结论 1、发现一个控制粒长的新主效QTL基因，其中KELCH结构域是OsPPKL1生物功能所必需。 2、水稻基因组中含有3个OsPPKLS基因，OsPPKL1和OsPPKL3负调控粒长，OsPPKL2正调控粒长。 3、qgl3基因产生较长粒长是可能是由于细胞数量的增加。 4、qgl3能够显著提高杂交种产量。 THANK YOU! * 穗粒数 千粒重 有效穗 粒形 灌浆 粒长 粒宽 粒厚 GW2 GW5 GS5 GW8 GS3 DEP1 粒形与产量 粒形与品质 粒形对稻米品质的形成具有重要影响 72.13 ± 2.32 g 17.80 ± 0.82 g 30.08 ± 1.34 ORF4 encodes a putative type 2A phosphatase (PP2A)with Kelch like repeat domain (PPKL) members. *