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Calibration Tutorial: Discovery32 The outline of this talk covers a brief backgrounder on the PDI laser optics bench, detector configurations, light scattering principles and approaches on how to maintain system cleanliness for optimum performance. In the applications section detector calibration with a protein standard and various chromatographic examples of biomolecular compounds separated with SEC diol columns are shown. The SEC columns vary in pore size and external column dimensions depending on the application. Two prototype columns are also included in the examples, a 1000 Angstrom pore size column for extending the separation range into the multi-million dalton range and narrow bore columns for reduction of mass loading requirements. The outline of this talk covers a brief backgrounder on the PDI laser optics bench, detector configurations, light scattering principles and approaches on how to maintain system cleanliness for optimum performance. In the applications section detector calibration with a protein standard and various chromatographic examples of biomolecular compounds separated with SEC diol columns are shown. The SEC columns vary in pore size and external column dimensions depending on the application. Two prototype columns are also included in the examples, a 1000 Angstrom pore size column for extending the separation range into the multi-million dalton range and narrow bore columns for reduction of mass loading requirements. The outline of this talk covers a brief backgrounder on the PDI laser optics bench, detector configurations, light scattering principles and approaches on how to maintain system cleanliness for optimum performance. In the applications section detector calibration with a protein standard and various chromatographic examples of biomolecular compounds separated with SEC diol columns are shown. The SEC columns vary in pore size and external column dimensions depending on the application. Two prototype columns are also include
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