Protein_SEC.pptVIP

Heinz GoetzWorld Wide Product ManagerLiquid Chromatography Protein Analysis by Aqueous Size Exclusion Chromatography(SEC) with the 1100 SEC System SEC: What is it? HPLC technique for analysis/characterization of macromolecules SEC: - w/ organic eluents (Gel Permeation Chromatogr.- GPC) - w/aqueous eluents (Gel Filtration Chromatogr. - GFC) GPC: - typical eluents: tetrahydrofuran, toluene, dimethylacetamide … - for synthetic macromolecules(plastics) GFC: - eluent: water, buffered water - biomacromolecules (proteins, poly-