從香草茶,绞股蓝提取的免疫刺激多糖的分离和鉴定.docxVIP

J. Agric. Food Chem. 2008, 56, 6905–6909 Isolation and Characterization of Immunostimulatory Polysaccharide from an Herb Tea, Gynostemma pentaphyllum Makino  6905 XINGBIN YANG,*,? YAN ZHAO,? YING YANG,?  AND  YUN RUAN? Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, and School of Pharmaceutical Sciences, Fourth Military Medical University, Xi’an 710032, China Water-soluble polysaccharide from Gynostemma pentaphyllum herb tea (PSGP) was isolated by hot- water extraction and ethanol precipitation. The chemical components and preliminary immunomodu- lating activity of PSGP were investigated both in vitro and in vivo. Capillary zone electrophoresis analysis showed that PSGP was a typical nonstarch heteropolysaccharide, with glucose being the main component monosaccharide (23.2%), followed by galactose (18.9%), arabinose (10.5%), rhamnose (7.7%), galacturonic acid (4.7%), xylose (3.9%), mannose (3.1%), and glucuronic acid (1.2%). PSGP could signi?cantly stimulate peritoneal macrophages to release nitric oxide, reactive oxygen species, and tumor necrosis factor-R in a dose-dependent manner. This immunostimulating activity of PSGP was further demonstrated by its inhibition on the proliferation of human colon carcinoma HT-29 and SW-116 cells incubated with the supernatant of PSGP-stimulated macrophage culture. It is evident that PSGP is a very important ingredient responsible for at least in part the immunomodulating activity of G. pentaphyllum herb tea. KEYWORDS: Gynostemma pentaphyllum herb tea; polysaccharide; capillary electrophoresis; immuno- stimulating activity INTRODUCTION The consumption of herb tea is popular in Asian countries. Gynostemma pentaphyllum Makino, named “Jiao-Gu-Lan” in Chinese, is a well-known herb tea in Asia. It is a saponin-rich plant and contains about 90 dammarane-type glycosides which are closely related to the compo