C3GRap1 和Dock180Rac1 信号通路在卵巢癌发病机制中的可能作用.docVIP

C3G/Rap1和Dock180/Rac1信号通路在卵巢癌发病机制中的可能作用( 高一萌1，张 瑜1，李文燕1，车亚玲1，王 瑾1，刘 斌2，王 辉1，令狐华1 （400016 重庆，重庆医科大学：附属第一医院妇产科1,基础医学院病理科2） [摘要] 目的 探讨鸟嘌呤核苷酸交换因子C3G/Rap1酶和鸟嘌呤核苷酸交换因子Dock180/Rac1酶信号通路在卵巢癌发病机制中的可能作用。方法 免疫印迹检测Dock180沉默的卵巢癌细胞SKOV3中C3G的表达，验证上皮性卵巢癌组织中Dock180与C3G的表达相关性；免疫组化比较卵巢癌组织中Dock180与C3G的表达趋势；免疫荧光观察SKOV3中Dock180与C3G及它们各自的下游蛋白Rac1/Rap1的定位。结果 Dock180基因沉默的细胞中C3G表达明显增强（P＜0.05）；Dock180与C3G在卵巢癌组织中的表达呈现相反趋势；C3G/Dock180均主要分布于胞质，下游效应蛋白Rap1/Rac1在胞膜和胞质都有表达，但Rap1以胞质为主，而Rac1可以伸展至胞膜及胞膜皱褶。 结论 卵巢癌细胞和组织中C3G与Dock180表达呈相反趋势，下游蛋白Rap1与Rac1在细胞内的定位分布差异，可能与C3G/Rap1和Dock180/Rac1信号通路在卵巢肿瘤发病机制中的不同作用有关。 [关键词] C3G； Rap1；人卵巢癌[中图法分类号]R 737.31 [文献标志码]A Potential role of C3G/Rap1 and Dock180/Rac1 pathway in the pathogenesis of ovarian cancer Gao Yimeng1, Zhang Yu1, Li Wenyan1, Liu Bin2, Che Yaling1, Wang Jin1, Wang Hui1, Linghu Hua1 （1Department of Obstetrics and Gynecology，First Affiliated Hospital Department of Pathology，Basic Medical College, Chongqing Medical University, Chongqing, 400016, China） [Abstract] Objective To investigate the potential role of C3G/ Rap1 and Dock180/Rac1 pathway in the ovarian cancer. Methods C3G expression was detected by western blotting in Dock180 knockdown ovarian cancer cell SKOV3. To validate the relationship between C3G and Dock180 expression in epithelial ovarian cancer ( EOC ) tissues. Immunohistochemical analysis was performed to measure the Dock180 and C3G expression pattern in ovarian cancer tissues. The location Dock180/C3G and Rac1/Rap1 was demonstrated in ovarian cancer cell SKOV3 by means of immunofluorescence. Results In Dock180 knockdown SKOV3 cells, C3G expression was found to be significantly increased as compared with that of WT, Empty.cont and NT.cont cells（P＜0.05）. C3G expression appears inversely related with that of Dock180 levels in EOC tissues（P＜0.05）. Both C3G and Dock180 are distributed around cytoplasm. Their downstream effectors, Rap1 and Rac1 are distributed both in plasmalemma and cytoplasm, with Rac1 distribution extending