遗传工程小鼠的制备繁育及应用 医学课件.pptxVIP

遗传工程小鼠的制备繁育及应用Story 1Timo “transgenic” mice (transgenic insertional mutants with obesity)Transgene insertionRegulatory locusMouse Ch2Transgene integration siteLCRC1RC2RC3BDNF/BdnfTimo “transgenic” mice~7kbSha, H., et al (,2007) Physiol Genomics. 31(2): p. 252-63第一个转基因小鼠（1982）by Richard Palmiter and Ralph Brinster Procedure of conventional TGThe “conventional” transgenesis is using short constructs with well-defined promoter regions.?第一个基因敲除小鼠（1987-89）2006年EUCOMM: 1.4亿欧元NorCOMM: 1 亿加元KOMP: 1.8亿美元ChCOMM: 1500万RMBCappechi2007年诺贝尔生理医学奖EvansSmithies基因组改造技术是建立疾病模型的核心ES target strategy123456Wild