CEA实时荧光定量RT.docVIP

CEA实时荧光定量RT 作者：葛明建,时德吴庆琛,王梅,李强,李良彬 【关键词】 胞肺癌患 【Abstract】 AIM: To assess whether surgical manoeuvre or resection of lung cancer could lead to haematogenous dissemination of malignant cells, to monito the quantity and timing of the shedding of lung cancer cells into the circulation of patients by fqRTPCR before, during and after surgery, and to determine the relationship between the sequence of vessel ligation and the haematogenous dissemination of cancer cells during operation. METHODS: Sixtynine peripheral blood samples were collected 1 d before operation, during operation and 7 d after operation from 23 consecutive patients with nonsmall cell lung cancer who underwent surgical resection with curative intention. All patients were randomly assigned before the operation to 2 surgical procedure groups according to the order of vessel ligation . Additionally, 10 patients with benign lung disease served as control subjects undergoing surgical resection. All the peripheral blood samples were subjected to fqRTPCR with carcinoembryonic antigen as marker. RESULTS: The level of CEA mRNA in peripheral blood ascended continuously within this period. The postoperative value was significantly higher than that of preoperation and that of operative day . There was a striking difference between adenocarcinoma and squamous carcinoma . The values of CEA mRNA in PB between PVfirst group and PAfirst group were significantly different . At the same time, there was a correlation between preoperative Tstage and perioperative CEA mRNA in PB . Among the 23 cases, 10 cases were positive . Both the control subjects and the volunteers were negative. CONCLUSION：A considerable proportion of patients who appear to have resectable nonsmall cell lung cancer might be regarded as having systemic disease, which is often undetectable by current tumour staging method. CEAexpressing tumour cells are disseminated mostly postoperatively, wh