两种工艺制备竹沥中游离氨基酸比较.docVIP

两种工艺制备竹沥中游离氨基酸比较 【关键词】nbsp; 烧制法；,,回流提取法；,,淡竹沥；,,游离氨基酸 　　摘要：目的比较传统烧制法和回流提取法两种方法制备的淡竹沥中游离氨基酸含量。方法采用柱前衍生HPLC法比较了两种不同方法制备的淡竹沥中游离氨基酸含量和种类。结果烧制法中含有天冬氨酸、丝氨酸和亮丝氨酸等15种氨基酸，而回流提取法中有7种氨基酸：苷氨酸、丙氨酸、酷氨酸、蛋氨酸、缬氨酸、苯丙氨酸、赖氨酸；回流提取法所得游离氨基酸总量较烧制法高出近12倍。结论该研究为竹沥制备工艺的改进奠定了基础。 　　关键词：烧制法；nbsp; 回流提取法；nbsp; 淡竹沥；nbsp; 游离氨基酸 　　Comparative Research on Free Amino Acid in DanZhuLi Prepared by Two Methods 　　Abstract：ObjectiveTo compare the kinds and contents of free amino acids in DanZhuLi prepared from baked process and refluxing process.Methods The kinds and contents of free amino acids in DanZhuLi prepared from two processes were analyzed by pre-column derivatization-high-performance liquid chromatography.Results The results showed that there were fifteen kinds of free amino acids including aspartic acid and leucine amino acid in DanZhuLi prepared by the baked process, while there were lack of seven kinds of free amino acids such as glycine, lsyine etc in DanZhuLi prepared by the fefluxing process. On the other hand, the contents of total amino acids in DanZhuLi prepared by the refluxing process was 12 times as large as the other method.ConclusionIt provieded foundation for improvement on preparation process. 　　Key words：Baked process;nbsp;nbsp; Refluxing process ;nbsp; DanZhuLi;nbsp;nbsp; Free amino acid nbsp;nbsp;nbsp; 　　禾本科刚竹属淡竹Phyllostachys nigra (Lodd.) Munro var. henonis (Mitf) Stapf ex Rendle除了应用于建筑和造纸等行业外；在医药方面有很重要的药用价值，它的秆用火烤灼流出的淡黄色澄清的液汁称为淡竹沥，药理上具有清热化痰，开窍定惊的作用，临床中用于治疗痰热咳嗽，痰黄气促，中风痰盛,惊风、破伤风等证［1］。据文献报道［2~4］，竹沥中主要含有氨基酸类、酚类、无机元素以及有机酸类成分，其中愈创木酚及氨基酸类成分是祛痰止咳作用的主要有效成分［5~7］。但是竹沥的质量易受天气变化、环境的影响，本研究采用HPLC法比较了不同方法制备淡竹沥中游离氨基酸的含量和种类，为竹沥制备工艺改进和药理作用研究提供科学依据。 　　1nbsp; 器材 　　1.1nbsp; 材料nbsp; 淡竹为禾本科刚竹属淡竹Phyllostachys nigra (Lodd.) Munro var. henonis (Mitford) Stapf ex Rendle，采自湖南长沙岳麓区，经湖南中医药大学药学院生药教研室周日宝教授鉴定。标本存于湖南中医学院中药教研室。 　　1.2nbsp; 仪器与试剂nbsp; 岛津高效液相色谱仪LC6A，RF530荧光检测器；LE80K型超速离心机；18 种氨基酸标准储备液；其他所用溶剂均为分析纯，流动相配制用色谱纯。 　　2nbsp; 方法与结果 　　2.1nbsp; 色谱条件 Hypersil ODS C18