亚硒酸钠对k562-adr多药耐药的逆转作用及其机制.docVIP

亚硒酸钠对K562/ADR多药耐药的逆转作用及其机制 nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;作 者：崔晶,丁璟,吴轶苹,刘复强,刘小超,王阳 【摘要】nbsp; 本研究探讨亚硒酸钠对白血病耐药细胞株K562/ADR细胞的多药耐药性的逆转作用及其逆转机制。 用噻唑蓝（MTT）法检测亚硒酸钠对K562/ADR细胞的生长抑制作用及其对K562/ADR细胞耐药性的逆转作用；应用流式细胞仪检测亚硒酸钠对K562及K562/ADR细胞凋亡率的影响；用半定量RTPCR法检测K562/ADR细胞中mdr1和bcl2基因的表达情况。 结果表明，10 μmol/L亚硒酸钠可以明显增加K562/ADR细胞对阿霉素敏感性，其耐药逆转倍数为2.31；早期凋亡率在10 μmol/L亚硒酸钠作用于K562细胞48小时是升高的；而中、晚期凋亡率在亚硒酸钠5、10 μmol/L作用48、72小时时均明显升高；两种浓度亚硒酸钠在作用48小时可使K562/ADR细胞早期凋亡率增加，作用48、72小时可使K562/ADR的细胞中、晚期凋亡率增高，10 μmol/L的亚硒酸钠所致凋亡率高于5 μmol/L，作用72小时的凋亡率高于48小时；亚硒酸钠可使K562/ADR细胞mdr1 mRNA及bcl2 mRNA表达下降。 结论： 亚硒酸钠可部分逆转K562/ADR细胞的耐药性，其机制可能是增加K562/ADR细胞凋亡率，下调mdr1 mRNA和bcl2 mRNA的表达。 【关键词】nbsp; 细胞凋亡 　nbsp;nbsp; Reversal Effect of Sodium Selenite on Multidrug Resistance in K562/ADR Cell Line andnbsp; Itsnbsp; Mechanisms nbsp;nbsp;nbsp;nbsp;nbsp; Abstractnbsp;nbsp;nbsp; This study was purposed to investigatenbsp; the reversal effect of sodium selenite on multidrug resistance in adriamycinresistant leukemic cell line K562/ADR and itsnbsp; mechanisms.nbsp; The cytotoxicity and the reversal effect of sodium selenite on K562/ADR cellsnbsp; were assayed by MTT method; thenbsp; apoptosis rate of K562 and K562/ADR cellsnbsp; were detected by flow cytometery, the mRNA expressions of mdr1 and bcl2 were measured by semiquantitative reverse transcriptase polymerase chain reaction (RTPCR).nbsp; The results showed that 10 μmol/L sodium selenite significantly increased the cytotoxicity of adriamycin to K562/ADR cell and the reverse index（RI）was 2.31;nbsp; the early apoptosis rate of K562 cells was elevated after treatment withnbsp; 5 μmol/Lnbsp; Na2SeO3nbsp; for 48 hours；and the mediumterm and late apoptosis rate was elevated after treatment withnbsp; both 5 and 10 μmol/Lnbsp; Na2SeO3nbsp; for 48nbsp; and 72 hours.nbsp; Both doses ofnbsp; 5 and 10 μmol/Lnbsp; Na2SeO3nbsp; increased the early apoptosis rate of K562/ADR at 48 hours，a