人胎盘与脐动、静脉血造血干-祖细胞归巢相关黏附分子表达的研究.docVIP

人胎盘与脐动、静脉血造血干/祖细胞归巢相关黏附分子表达的研究 nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 作者：苏蕊，陈代雄，方宁，陈琦，龚芳泽 【摘要】nbsp; 为了评价人胎盘组织造血干/祖细胞（hematopoietic stem/progenitor cell，HSPC）的归巢能力，采用机械法制备人胎盘组织单个细胞悬液，用流式细胞术分析胎盘组织及脐动、静脉血有核细胞中CD34+细胞及其亚群的含量，检测三者来源的CD34+细胞表面归巢相关黏附分子CD44、CD11a、CD62L、CD49d、CD49e和CD54的表达水平。结果显示，胎盘组织CD34+细胞及CD34+CD38-细胞百分率明显高于脐动、静脉血；脐动脉与脐静脉血中HSPC百分率没有明显差异。胎盘来源CD34+细胞高度表达黏附分子CD11a、CD49d、CD44、CD49e及CD54，其中表达CD49e及CD54水平明显高于脐动、静脉血CD34+细胞。胎盘来源的CD34+CD62L+细胞百分率为(64.58±15.52)%，低于脐静脉血来源的表达。结论：人胎盘富含HSPC。胎盘来源的CD34+细胞多数黏附分子的表达水平近似或高于脐血，提示胎盘HSPC的归巢能力有可能强于脐带血。 【关键词】nbsp; 胎盘 　　Expressions of Homing-Related Adhesion Molecules in Hemato-poietic Stem/Progenitor Cells Derived from Human Placenta，Umbilical Cord Arterial andnbsp; Venous Blood 　　Abstractnbsp; The aim of this study was to evaluate the homing capabilities of hematopoietic stem/progenitor cells (HSPCs) derived from human placenta tissues (PT). Single cell suspension of human PT was prepared by mechanical method. The expression levels of homing-related adhesion molecules (HRAM) including CD11a，CD49d，CD44，CD49e，CD62L and CD54 on CD34+ cells and the percentages of CD34+ cells and their subpopulations in nucleated cells (NC) from fresh human PT，umbilical cord arterial blood (UCAB) and umbilical cord venous blood(UCVB) were detectednbsp; by using flow cytometry. The results showed that the percentage of CD34+cells and CD34+CD38-nbsp; cells in placenta were higher than those in UCAB andnbsp; UCVB. There were no significant difference in percentage of HSPC between UCAB and UCVB. Placenta-derived CD34+ cells strongly expressed CD11a，CD49d，CD44，CD49e and CD54，among whichnbsp; expression levelsnbsp; of CD49e and CD54 on placenta-derived CD34+ cellsnbsp; were significantly highernbsp;nbsp; than those on UCAB and UCVB-derived CD34+ cells. While the percentage of CD34+CD62L+ cells in placenta was only lower than that in UCVB. It is concluded that human placenta i