ppt课件-measurementofbioreactorkla-auburnuniversity.pptVIP

Measurement of BioreactorKLa Motivations 2. Good example of mass transfer at gas-liquid interface 3. Experience modeling in both semi-empirical and factorial methods Types of Products Natural Products Drugs Penicillin is early example Taxol Mupricin Cyclosporin A, etc. Foods Fermented beverages Fermented dairy products Types of Products Transgenic Products Gene for a therapeutic protein inserted in foreign expression system Factor IX a-1-antitrypsin EPO Antibodies antithrombin III tissue plasminogen activator (TPA) Interferons, etc. Expression Systems Bacterial Cells Fungal Cells Plant Cells Insect Cells Mammalian Cells Types of Bioreactors (fermenter)(often depends on shear sensitivity) Stirred tank Aerobic or Anaerobic (air-sparged if aerobic) Most common for bacterial cells Bubble or airlift column Good for shear-sensitive cells Fixed bed systems Trickle beds, hollow membrane fiber (mammalian cells), etc. Industrial Stirred Fermenter Experimental Apparatus Transport in Bioprocess Systems Why is KLa Important? Dissolved oxygen is an important substrate in aerobic fermentations. Since oxygen is sparingly soluble in water, it may be the growth-limiting substrate in these fermentations. For bacteria and yeast cultures, the critical oxygen concentration is about 10% to 50% of the saturated DO (dissolved oxygen concentration). Equation for Transport Terms affecting rate KLa What we are trying to determine and correlate with mixing speed and aeration rate Two quantities multiplied together Liquid side (essentially overall mass transfer coefficient) Total area of bubbles in bioreactor Can’t be separated Some Interactions Affecting Oxygen Transport in Aerobic Systems Terms affecting rate C* (saturation oxygen concentration; max solubility of the gas in liquid) - Constant at a given T and P - Available in tables (see on-line lab manual) CL (C(t)) the oxygen concentration at a given time during the run; what we measure - {C*- CL} = “dr