a b s t r a c t- bryn mawr college（b s t r c t -布林莫尔学院）（61页）.docVIP

ABSTRACT Intercalating molecules can distort the helical shape of DNA, thereby inhibiting replication enzymes and in turn have pharmaceutical applications. Due to their inherent photochemical stability, as well as redox and photophysical properties, transition metal complexes make it possible to utilize multiple techniques to study intercalation. One such complex, [Ru(phen)2DPPZ]2+ has been shown in previous work to intercalate due to its large heterocyclic ring system. Previous fluorescence and absorbance titrations give evidence that four similar ruthenium compounds, [Ru(II)(bpy)2(phen-pterin)]2+, [Ru(II)(bpy)2(phen-alloxazine)]2+, [Ru(II)(bpy)2(phen-dimethylalloxazine)]2+, and [Ru(II)(bpy)2(phen-diaminopteridine)]2+ bind to calf thymus DNA. These studies cannot specify the mode of binding, so additional experiments are required. Reported are the results of viscometry, plasmid unwinding, thermal denaturation and circular dichroism, studies which can differentiate between binding mechanisms. The combination of experimental data from each method show that [Ru(bpy)2(phen-dimethylalloxazine)]2+ , [Ru(bpy)2(phen-alloxazine)]2+ and [Ru(bpy)2(phen-diaminopteridine)]2+ do bind to DNA via an intercalative mode. Additional study of these compounds leading to further characterization of binding could result in the development of stable complexes for use as DNA probes and the discovery of life saving pharmaceuticals. TABLE OF CONTENTS Abstract 1 Acknowledgements 3 Introduction Modes of Binding 4 Metallointercalators 7 Oxidative Reactions 11 Ruthenium Intercalators 13 New Ruthenium Complexes 27 Our Contribution to DNA Binding Characterization of Ruthenium(II) Pteridinyl Compounds 32 Results 34 Discussion 45 Materials 56 Methods 57 Works Cited