chapter7 f e c a l c o l i f o r m s- pennsylvania d e p（第7章f e c l c o宾夕法尼亚州l i f o r m s - d e p）.docVIP

Chapter 7 TABLE OF CONTENTS Fecal Coliform Page Section 1: General 2 Section 2: Introduction to Fecal Coliform Testing 2 Section 3: Glossary 2-3 Section 4: Approved Methods 3 Section 5: Safety and Hygiene 3 Section 6: Equipment and Reagents 4-6 Section 7: Preparation of Glassware 6 Section 8: Sterilization 6-7 Section 9: Serial Dilution Procedure 7 Quiz 7.1 8 Section 10: Bacteriological Sampling 8 Section 11: Sample Dechlorination 8 Section 12: Sampling Procedures 8-12 Quiz 7.2 12 Section 13: Fecal Coliform Confirming Test 12-14 Section 14: Sample Calculation 15-16 Quiz 7.3 15 Section 15: Interferences 16-17 Section 16: Preparation of Dilution Water 17-18 Section 17: Colony Counting 19-20 Quiz 7.4 21 Section 18: Comparison of Test Methods 21-23 Quiz 7.5 23 Section 19: QA/QC 23-24 Answers to Quizzes 25-27 Appendix A: References Appendix B: Geometric Mean Calculation Appendix C: Sample Bench Sheets Appendix D: Methods Checklist Chapter 7 FECAL COLIFORMS Section 1: GENERAL As in any type of industry, some form of process monitoring is required to ensure that the quality of the final product is maintained at the highest possible level. In the wastewater treatment industry, laboratory testing is one of the methods used to ensure that a high quality effluent is maintained. Accurate and reliable laboratory analyses are absolutely necessary to monitor effluent characteristics and to provide a basis for making operational changes in the treatment process itself. Section 2: INTRODUCTION TO FECAL COLIFORM TESTING Fecal coliform bacteria are nondisease causing organisms which are found in the intestinal tract of all warmblooded animals. Each discharge of body wastes contains large amounts of these organisms. The presence of fecal coliform bacteria in a stream or lake indicates the presence of human or animal wastes. The number of fecal coliform bacteria present is a good indicator of the amount of polluti