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NORTHWEST MISSOURI STATE UNIVERSITY ABSTRACTS OF COMPLETED RESEARCH PROJECTS APPROVED BY FACULTY RESEARCH COMMITTEE PERIOD 2002-2006 September 25, 2006 Northwest Missouri State University budget grants for Faculty Research began in 1974. Since that time, approximately 317 research proposals have been funded. Since the last report in December 2002, 35 research projects have been completed and submitted to the Graduate Office. Nathan Paul, Graduate Assistant in the Graduate Office, compiled this report of abstracts submitted by the researchers. The authors, titles, dates funded and abstracts are as follows: Abstracts “Characterization of factor(s) present in Bittermelon (Momordica charantia) that cause delay of tumor development in line 69-2F wap-ras mice”, and “Effects of Tamoxifen, Vitamin E, Interleukin-2 or their combination on the development of tumor in line 69-2F wap-ras mice.” “Characterization of Powdery Mildew disease resistance factor(s) in Cucumis melo var. Sweet Thing hybrid.” PI: Alejandro Ching Jr., CoPI: Rafiq Islam CoPI: David Smith From bittermelon seeds, using Cibacron blue matrix we were able to purify a protein that was determined to be 30 kDa by SDS. Characterization of this protein as the facor for tumor delay was not complete as Dr. Smith, who was working on this part of the project, left for a year on sabbatical. The following year when he returned, he decided to retire and take a teaching position at a medical school on the Caribbean. We isolated mitochondria from the seeds of Cucumis melo var. Sweet Thing hybrid as well from the parents. Following isolation, the DNA from each variety was PCR amplified using 20 random primer sets from Operon. Analysis of the PCR amplified products from each variety showed differences in a number of DNA bands from the random primer set 6, 8, 10 and 15. The different DNA bands were isolated from the gel, but were not enough to se