肾缺血预处理对兔未成熟心肌bcl.docVIP

肾缺血预处理对兔未成熟心肌Bcl 作者：孙忠东，高尚志，池一凡，夏家红，杨辰垣 【关键词】 缺血预处理 Effects of renal ischemic preconditioning on the expressions of Bcl2, Bax, Fas proteins in immature myocardial cells in rabbits 　　【Abstract】 AIM: To investigate the effects of renal ischemic preconditioning (RIP) on apoptosis and expressions of Bcl2, Bax and Fas proteins in immature myocardial cells in rabbits. METHODS: Isolated working rabbit heart model was used in this study and 24 immature rabbits were randomly divided into control group, ischemia/reperfusion (I/R) group and RIP group. The TUNEL, nucleosomal ladder of DNA fragments and the expression of Bcl2, Bax and Fas proteins were detected. RESULTS: Compared with that in I/R group, TUNEL cell apoptosis was lower ［(4.19±1.13)% vs (12.30±2.10)%, Plt;0.01］, ladder of DNA fragments was lower (57 350±1765 vs 135 200±3370, Plt;0.01), Bcl2 expression was higher (33 525±1026 vs 12 385±860, Plt;0.01), Bax expression was lower (8640±768 vs 32 472±1128, Plt;0.01) and Fas expression was lower (8936±912 vs 32 100±1260, Plt;0.01) in RIP group. CONCLUSION: RIP decreases immature myocardial cell apoptosis and affects the expressions of Bcl2, Bax and Fas proteins. 　　【Keywords】 kidney; ischemic preconditioning; apoptosis; Bcl2; Bax; Fas 　　【摘要】 目的： 探讨肾缺血预处理(RIP)对兔未成熟心肌细胞凋亡和Bcl2, Bax, Fas蛋白表达的影响. 方法： 24只幼兔采用Langendorff离体心脏灌注模型，随机分为正常对照组（C）、心脏缺血/再灌注组(I/R)和肾缺血预处理组(RIP)，每组8只，测定各组未成熟心肌细胞凋亡和Bcl2, Bax, Fas蛋白表达. 结果： RIP组与I/R组比较，心肌细胞凋亡率明显减少［(4.19±1.13)% vs （12.30±2.10）%, Plt;0.01］，DNA片段梯光密度明显减少(57 350±1765 vs 135 200±3370, Plt;0.01), Bcl2表达增多(33 525±1026 vs 12 385±860, Plt;0.01), Bax(8640±768 vs 32 472±1128, Plt;0.01)和Fas(8936±912 vs 32 100±1260, Plt;0.01)表达明显减少. 结论： RIP可减少缺血再灌注后兔未成熟心肌细胞凋亡和影响Bcl2, Bax, Fas蛋白的表达. 　　【关键词】 肾；缺血预处理；细胞凋亡；Bcl2；Bax；Fas 　　0引言 　　实验表明，成熟心肌和心肌外的组织器官缺血预处理可减少心肌细胞凋亡的发生，其中Bcl2, Bax, Fas等基因蛋白的表达在细胞凋亡中起重要作用. 肾缺血预处理(renal ischemic preconditioning ,RIP)对未成熟心肌细胞凋亡是否有影响的报道甚少. 本实验目的是观察RIP对兔未成熟心肌细胞凋亡和Bcl2, Bax, Fas等基因蛋白表达的影