大叶冬青叶中2个新的三萜皂苷.docVIP

大叶冬青叶中2个新的三萜皂苷 　　[摘要] 大叶冬青为苦丁茶的主要基源植物，三萜皂苷为其主要的活性和特征性成分。该研究从大叶冬青叶中分离得到了2个新的三萜皂苷类化合物，大叶冬青皂苷 R （1）和大叶冬青皂苷S （2），并根据NMR，MS等波谱方法和理化性质鉴定了其化学结构。其中大叶冬青皂苷R为第1个含有1928内酯环的三萜皂苷。 　　[关键词] 冬青科； 大叶冬青； 三萜皂苷 　　[Abstract] Two new triterpenoid glycosides， latifolosides R and S （1 and 2）， were isolated from the leaves of Ilex latifolia by various column chromatographic methods. Their structures were elucidated based on NMR spectroscopic data and chemical evidence. 　　[Key words] Aquifoliaceae； Ilex latifolia； triterpenoid glycoside 　　doi：10.4268/cjcm 　　The leaves of Ilex latifolia Thunb. （Aquifoliaceae）， a Chinese bitter tea called as KuDingCha， have been widely consumed as health beverage in China for thousands years. In traditional Chinese medicine， the leaves are also used for the treatment of dysentery， sore throats， obesity， hypertension as well as various inflammatory diseases[1]. Flavonoids， polyphenolics， triterpenes and triterpenoid glycosides have been isolated and identified from the leaves of I. latifolia[27]. Among them， triterpenes and triterpenoid glycosides were considered to be the major and characteristic constituents of this plant， some of which showed antioxidant[8]， antibacterial [9] and lipidlowering[1011] activities. Our previous papers[7，1112] reported the isolation and structural identification of several new triterpenes and triterpenoid glycosides from the title plant. As a part of our continuing investigation， two new triterpenoid glycosides， latifolosides R and S （1 and 2） were further isolated from the same plant （Fig. 1）. This paper describes the isolation and structural elucidation of two new compounds. 　　Fig.1 Chemical structures of 1 and 2 　　1 Material 　　1.1 General experimental procedures Optical rotations were measured with a JASCO P1020 polarimeter. UV data were obtained on a JASCO V550 UV/vis spectrophotometer. IR spectra （KBr） were recorded on a JASCO FT/IR480 Plus Fourier Transform spectropolarimeter. 1H and 13C NMR spectra wer