HIF1αVEGFC在非小细胞肺癌中的表达及其与淋巴转移的关系.docVIP

HIF-1α、VEGF-C在非小细胞肺癌中的表达及其与淋巴转移的关系 　　作者：丁彦光　 达嘎 李勇　武建英 作者单位：1.青海大学医学院;2.青海大学附属医院心胸外科 　　【摘要】 目的 探讨低氧诱导因子-1Α(HIF-1Α)、血管内皮生长因子-C(VEGF-C)在非小细胞肺癌(NSCLC)中的表达及其与淋巴转移的关系。方法 采用PV-9000免疫组织化学法检测58例NSCLC和20例癌旁对照肺组织中HIF-1Α、VEGF-C的表达情况，并记数VEGFR-3标记淋巴管内皮细胞的微淋巴管密度(LMVD)。结果 HIF-1Α和VEGF-C在NSCLC组的阳性表达率分别为65.5%和60.3%，明显高于癌旁对照肺组织 (P 0.05 );NSCLC组织中HIF-1Α和VEGF-C的表达的高低与有无淋巴结转移、TNM临床分期密切相关(P0. 05)，而与性别、年龄、病理组织类型以及肿瘤的分化程度无显著相关(P 0.05);LMVD在NSCLC组和癌旁对照组计数分别为6.6±2.4和2.9±1.7，差异有统计学意义(P0.05);HIF-1Α与VEGF-C的表达呈明显相关(rpearson=0.464，P 0.05)，HIF-1Α与LMVD呈正相关(rs = 0.506，P 0.05)，VEGF-C与LMVD也呈正相关(rs = 0.608，P 0.05)。结论 HIF-1Α及其靶基因VEGF-C在NSCLC中的表达明显上调， HIF-1Α可能通过诱导VEGF-C的过表达参与NSCLC的淋巴管生成，在NSCLC的发生、发展以及转移过程中发挥着重要作用。 丁彦光(1982～)，男，汉族，山东籍，青海大学医学院2006级硕士研究生 　　【关键词】 非小细胞肺癌 HIF-1Α VEGF-C 微淋巴管密度 免疫组织化学 淋巴转移 　　EXPRESSION OF HIF-1Α AND VEGF-C AND THERELATIONSHIP OF LYMPHATIC METASTASISIN NON SMALL CELL LUNG CANCER 　　Ding Yanguang1，Da Ga2，Li Yong2，Wu Jianying2 　　(1. Medical College of Qinghai University; 　　2 .Department of cardiothoracic surgery, the Affiliated Hospital of Qinghai University) 　　Abstract Objective To investigate the expression of HIF-1Α、VEGF-C and their relation to lymphangiogenesis and lymph node metastasis in non small cell lung cancer(NSCLC). Methods The expressions of HIF-1Α and VEGF-C in 58 cases of NSCLC tissues and 20 cases of the adjacent tissues were detected by PV-9000 immunohistochemical technique. LMVD was determined by VEGFR-3 immunostaining. Results The positive rate of HIF-1Α and VEGF-C expression in NSCLC was 65.5% and 60.3%,which is significantly higher than that in the adjacent tissues (P0.05 ). There was a significant difference of HIF-lΑ and VEGF-C protein expression between NSCLC tissues and the adjacent tissues (P0.01).The expression of HIF-1Α and VEGF-C were both correlated with lymph node involvement (P0.05) and TNM clinical stage (P0.05),but no significant correlation between sex, age, pathological type and differentiation(P 0.05).The expression