Effects of Alcoholic Dilutions on Bacterial Growth酒精稀释液对细菌生长的影响.pptxVIP

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2017-06-15 发布于上海
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Effects of Alcoholic Dilutions on Bacterial Growth酒精稀释液对细菌生长的影响.pptx

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Project by: Lauren Pease Grade 9 Effects of Alcoholic Dilutions on Bacterial Growth Problem What effect do different dilutions of alcohol have on bacterial growth? Does alcohol have a greater inhibitory effect on the growth of gram-negative or gram-positive bacteria? Research of Bacterial Species Staphylococcus epidermidis is found on the outer layer of the human skin and is the leading pathogen of foreign body infections. -it is typically immune to most antibiotics Escherichia coli lives in the intestinal tract of the human body and many other animals. Research of Gram-negative vs. Gram-positive Bacteria Gram-positive bacteria -cell wall contains a thick layer of peptidoglycan that encompasses the entire cell wall Gram-negative bacteria -cell wall contains a thin layer of peptidoglycan located only between the cytoplasmic and outer membrane Peptidoglycan is a polymer that consists of glycan and peptide chains which is responsible for inflexibility of cell walls in prokaryotes Research of Ethanol Alcohol Often referred to as “pure alcohol” Ethyl alcohol without additives or denaturants Is a group of chemical compounds whose molecules contain a hydroxyl group bonded to a carbon atom Made by the fermentation of sugars Hypothesis If there is a higher percentage of alcohol content, the alcohol will greater inhibit the growth of the bacteria. Also, if there is an equal percentage of alcohol used on the Staphylococcus Epidermidis and Escherichia Coli, then the Staphylococcus Epidermidis will be greater affected by the alcohol. Materials 5 Petri dishes of Staphylococcus epidermidis (gram-positive bacteria) 5 Petri dishes of Escherichia coli (gram-negative bacteria) 4 different dilutions of ethanol alcohol at 10 mL each with dilutions of 25%, 50%, 75%, and 100% 40 sterile disks submerged in the dilutions of ethanol alcohol (4 on each dish) Sharpie Tape Goggles and gloves were used for safety Variables Independent Variables -dilutions of ethanol alcohol Dependen