双向电泳蛋白质组技术在残胃癌组织研究中的应用.docVIP

双向电泳蛋白质组技术在残胃癌组织研究中的应用 　　[摘要] 目的 探讨双向电泳蛋白质组技术在残胃癌组织研究中的应用效果。 方法 选取3对残胃癌及癌旁正常胃黏膜组织，利用双向电泳蛋白质组技术对其进行研究。 结果 获得了质量良好的胃癌组织蛋白质组双向电泳图谱。分析之后可得，癌旁组织平均984个蛋白点，残胃癌组织凝胶平均1068个蛋白点。一共有112个差异表达的蛋白点，其中，显著差异（表达差异2倍以上）的蛋白点42个。在这42个蛋白点之中，在残胃癌组织里表达上调的一共有17个点；在残胃癌组织里表达下调的一共有14个点；在残胃癌组织中失表达的一共有8个点；仅在残胃癌组织中表达的一共有3个点。 结论 利用双向电泳蛋白质组技术对残胃癌组织进行研究可以获得良好的应用效果。 　　[关键词] 残胃癌；蛋白质；双向电泳蛋白质组技术 　　[中图分类号] R735.2 [文献标识码] A [文章编号] 1673-7210（2014）08（c）-0020-05 　　Application of two-dimensional gel electrophoresis proteomics in residual gastric cancer tissue study 　　SUN Yusheng1 ZHU Guanbao2 TAO Lijun1 FANG Jun1 XU Hongbo1 LIN Cai3 　　1.Department of Traumatology， the First Affiliated Hospital of Wenzhou Medical University， Zhejiang Province， Wenzhou 325000， China； 2.Department of General Surgery， the First Affiliated Hospital of Wenzhou Medical University， Zhejiang Province， Wenzhou 325000， China； 3.Department of Burns， the First Affiliated Hospital of Wenzhou Medical University， Zhejiang Province， Wenzhou 325000， China 　　[Abstract] Objective To explore the application effect of two dimensional electrophoresis proteomics techniques in the study of residual gastric cancer tissue. Methods 3 pairs of residual gastric cancer and adjacent normal gastric mucosa tissues were selected， and were studied by two-dimensional electrophoresis technology in proteomics. Results Gastric tissue of two-dimensional gel electrophoresis profiles of good quality was obtained. The average of protein spots of adjacent mucosa were 984， the residual gastric cancer tissue were 1068 after the analysis of available. There were 112 protein spots differentially expressed in total， and 42 protein spots were significantly different （expressed more than 2 times）. There were 17 protein spots in total raised in gastric cancer tissue； 14 protein spots in total cutted； 8 protein spots in total loss of expression； and 3 protein spots in total expressed only in the residual gastric cancer tissue among 42 protein spots. Conclusion Applica