人参皂苷Rb1拮抗肝癌细胞抑制NK细胞免疫功能的研究.docVIP

白消安与人参皂苷Rb11 陈红霞2△ (1△湖北中医药大学检验学院2△湖北科技学院基础医学院病理生理教研室) 摘要：目的 探讨化疗药物白消安与中药人参皂苷Rb1协同促进肝癌细胞HepG2)分泌HSP70，进而增强NK细胞杀伤功能的免疫调节作用。方法HepG2经过白消安、人参皂苷Rb1以及白消安联合人参皂苷预处理后分别收集培养上清，其对照为未经过药物处理的HepG2培养上清。通过ELISA方法检测上清中HSP70的表达水平；并将上清分别作用于NK细胞，随后检测其对靶细胞的杀伤率。结果HepG2的培养上清能显著抑制NK细胞的杀伤功能；但是经过白消安、人参皂苷Rb1以及白消安联合人参皂苷预处理的HepG2的培养上清作用于NK细胞后，NK细胞杀伤功能显著恢复，其中以白消安联合人参皂苷组的作用最显著(P0.05)，同时NK细胞杀伤功能的恢复程度与上清中HSP70的浓度呈正相关(P0.01)。结论 肝癌细胞(HepG2)的培养上清对于NK细胞的杀伤功能有免疫抑制效应。白消安和人参皂苷Rb1能拮抗这种免疫抑制，二者联合应用有协同效应，其机制可能与增强癌细胞分泌免疫调节物质HSP70有关，提示适当联用化疗药物与传统中药能更有效的提高NK细胞的抗癌效应从而发挥免疫调节作用。 关键词 肝癌细胞；自然杀伤细胞；白消安；人参皂苷Rb1；HSP70 [中图分类号] 　R284 [文献标识码] 　A Busulfan and ginsenoside Rb1 synergetically cause increased release of HSP70 from human hepatocellular carcinoma cell line HepG2 that elicit enhanced natural killer cell anti-tumor responses in vitro WANG Lei 1 CHEN Hongxia2△ (1Institute of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan Hubei 430065,China; 2△ Department of Pathophysiology, Hubei University of Science and Technology, Xianning Hubei 437100, China;) Abstract Objective: To explore whether the busulfan and ginsenoside Rb1 can enhance the release of heat shock protein 70 (HSP70) synergetically from human hepatocellular carcinoma cell line (HepG2) that elicit an effect on the upregulation of the cytotoxicity of natural killer (NK) cells. Methods: HepG2 were pre-treated with busulfan, ginsenoside Rb1, or busulfan and ginsenoside Rb1. The HepG2 without any pre-treatment was used as a control. The levels of HSP70 in the supernatants were assayed by ELISA respectively. Then, the effects of the collected HepG2 supernatants on the cytotoxicity of NK cells were assessed. Results: The collected supernatant of HepG2 showed inhibitory effects on the cytotoxicity of NK cells. However, when HepG2 were pre-treated with busulfan and (or) ginsenoside Rb1, the down-regulated effects of HepG2 supernatant could be significantly reversed. And the effect was significantly strongest when HepG2 was pre-treate