花瓣中荧光色素分析.doc

杜鹃（Rhododendron simsii）花瓣中荧光色素提取及应用初探 杨燕 陆小平 (金螳螂建筑与城市环境学院 08级城市园艺) 摘要 用DiaionHP-20和SephadexLH-20对杜鹃花瓣中色素进行了分离和纯化，获得了高浓度的植物源性荧光物质，并对荧光物质进行了抑菌试验和防伪标识试验。结果表明，花瓣浸提液中的色素经SephadexLH-20，可以清晰在看到5种不同荧光区域，其中绿黄绿色荧光效果最为明显。抑菌试验显示，该荧光色素对双球菌生长有很好的抑制作用；因此，对植源性荧光色素进行深入研究，不仅可以应用于新鲜食品的抑菌保鲜，减少化学防腐物质的滥用，而且可以开发成为新型防伪印刷材料---水性天然荧光油墨油墨Preliminary study on the extraction and application of fluorochrome in Rhododendron simsii’ petals YANG Yan, ZHAO Ge, LU Xiao-ping （Gold Mantis school of Architecture and Urban Environment, 08 Urban Horticulture） Abstract: By using DiaionHP-20 and SephadexLH-20 to isolated and purificated the pigment of Rhododendron simsii, a high concentration of fluorescent substances from plant organs was obtained, and then study on the antibacteria test and anti-counterfeit marking test was carried out. The result reveals that though extracting the pigment in the petals by SephadexLH-20, 5 different fluorescence regions can be clearly see, of which the yellow-green region was the most obvious. The result of antibacteria test shows that the fluorochrome could inhibit the growth of diplococcus. Thus the deeper research of the fluorochrome from plant organ could not only be used in the antibacterial activity of fresh food preservation aiming at preventing the abuse of chemical preservation substance, but also develop a new type of anti-counterfeit printing materials---natural water-based fluorescent ink, and the result brings business opportunities to the new industry of “green anti-counterfeit printing”. Key words: Rhododendron simsii; fluorochrome; antibacterial; fluorescent ink 杜鹃花的色、形、香、姿、韵兼备，是中国十大名花之一[1]。杜鹃花属有600余种分布于我国，占全世界种类的59％[2]。杜鹃花一般春季开花，花色有白、粉白、白绿、黄、乳黄、橙黄、金黄到红、粉红、桃红、金红、大红、深红、玫瑰红、紫、深紫以及红白相间等多种颜色。因此，杜鹃花是开发天然花色素的良好资源[3]。 植源性荧光色素是指植物体中的杂多环化合物黄酮类化合物，经紫外线照射后能发荧光，它不同于化学合成的荧光染料无毒、无辐射物质可荧光分自发荧光诱发荧光两种，如叶绿素等经紫外线照射后发出红色荧光种黄酮类经紫外线照射发出蓝、黄色荧。黄酮类的Rhododendron simsii）花瓣取自苏州大学独墅湖校区，于35±1℃的烘箱内烘干新鲜花瓣后置干燥箱内保存备用。双球菌菌液（OD值为2.5左右）由苏州大学医学部郑小坚博士惠赠。 实验试剂与设备 大孔吸附树脂DiaionHP-20（日本），