DNA甲基化和组蛋白修饰在克隆动物发育过程中的作用要素.pdf

HEREDITAS (Beijing) 2010 8 , 32(8): 762―768 ISSN 0253-9772 DOI: 10.3724/SP.J.1005.2010.00762 综述 DNA 甲基化和组蛋白修饰在克隆动物发育过程中的 作用 郭磊 1, 李慧 1, 2, 韩之明 1 1. 2. , 100875 , 100101; 体细胞核移植在农业应用、生产疾病模型动物、转基因家畜或产生人胚胎干细胞来治疗人类的疾病方面 有巨大的应用潜力。虽然已经成功克隆出多种哺乳动物, 但该技术仍存在一些未解决的问题, 包括产生克隆动 物的效率低和克隆动物的异常等。异常的表观遗传重编程是克隆胚胎发育失败的一个重要因素。文章重点论述 了 DNA 甲基化、组蛋白修饰及其与克隆胚胎发育的关系。了解表观遗传调控机制有助于解决核移植技术中存 在的问题, 有利于更好地应用这项技术。 体细胞核移植; 表观遗传修饰; DNA 甲基化; 组蛋白修饰 Effect of DNA methylation and histone modification during the development of cloned animals GUO Lei1, LI Hui1, 2, HAN Zhi-Ming1 1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; 2. College of Life Sciences, Beijing Normal University, Beijing 100875, China Abstract: Somatic cell nuclear transfer (SCNT) has great potential for agricultural applications, generation of medical model animals, transgenic farm animals or generating human embryonic stem cells for treatment of human diseases. Cloned animals derived from somatic cells have been generated in several mammal species, but there are still some unsolved problems with current cloning technology, for example, the low efficiency of animal cloning and the abnormal development of cloned animals. One critical factor of these developmental failures of cloned embryos is the aberrant epigenetic reprogramming. This review focuses on DNA methylation and histone modifications and the relationship between these two epigenetic modifications and the development of cloned embryos. Understanding the mechanisms of epigenetic regulation will be useful to solve the technical problems of SCNT and enable better applications of this technology. Keywords: somatic cell nuclear transfer; epigenetic modification; DNA methylation; histone modifications , [1] Dolly [2] [3], 收稿日期: 2009?11???; 修回日期: 2010?03?19 基金项目: ( 作者简介: (1984?), , , 通讯作者: (1974?), , ,, (863 )( 2008AA101006) Tel: 010 E-mail: guolei@ Tel: 010 E-mail: hanzm@ , 8 : D