Optical properties of parietal peritoneum in the spectral光谱中壁层腹膜的光学特性.pptVIP

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Optical properties of parietal peritoneum in the spectral光谱中壁层腹膜的光学特性.ppt

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Optical properties of parietal peritoneum in the spectral光谱中壁层腹膜的光学特性

Optical properties of parietal peritoneum in the spectral range 350-2500 nm Marina D. Kozintseva1, Alexey N. Bashkatov1, Elina A. Genina1, Vyacheslav I. Kochubey1, Sergey Yu. Gorodkov2, Dmitry A. Morozov3, Valery V. Tuchin1 ? 1 Department of Optics and Biophotonics of N.G. Chernyshevsky Saratov State University, Saratov, Russia 2?Saratov?State?Medical?University?named?after?V.I.?Razumovsky 3?Moscow State Scientific-Research Institute of Pediatrics and Children Surgery Motivation: The wide application of optical methods in modern medicine in the areas of diagnostics, therapy and surgery has stimulated the investigation of optical properties of various biological tissues. The knowledge of tissue optical properties is necessary for the development of the novel optical technologies of photodynamic and photothermal therapy, optical tomography, optical biopsy, and etc. Numerous investigations related to determination of tissue optical properties are available however the optical properties of many tissues have not been studied in a wide wavelength range. Materials and Methods: For this study 13 samples of the parietal peritoneum mucous membrane , 10 samples of the parietal peritoneum muscle membrane and 14 samples of the entire parietal peritoneum (mucous membrane + muscle membrane) have been used. The samples keep in saline during 3-4 hour until spectrophotometric measurements at temperature 4-5°C. All the tissue samples has been cut into pieces with the area about 8.4 ? 0.99 cm2. For mechanical support, the tissue samples have been sandwiched between two glass slides. The average thickness of the samples was 0.77 ? 0.2 mm for parietal peritoneum mucous membrane, 2.3 ? 0.8 mm for parietal peritoneum muscle membrane and 3.13 ? 0.15 mm for entire parietal peritoneum (mucous membrane + muscle membrane). Measurement of the diffuse reflectance, total and collimated transmittance have been performed using a commercially available spectrophotometer PerkinElmer LAMBDA 950 i