PowerPoint 演示文稿 Bioinformatics Division Center PowerPoint演示文稿生物信息学分中心.pptVIP

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PowerPoint 演示文稿 Bioinformatics Division Center PowerPoint演示文稿生物信息学分中心.ppt

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PowerPoint 演示文稿 Bioinformatics Division Center PowerPoint演示文稿生物信息学分中心

Summary Defining oocyte/sperm contributed DMR by linear regression is indirect Can’t cover much non-CpGs Why oocyte-contributed DMR in HCP but sperm-contributed DMR in intergenic regions? A model for DNA methylation dynamics during early embryogenesis Harvard University Department of Stem Cell and Regenerative Biology, Broad Institute Rudolf Jaenisch at the Whitehead Institute,MIT, one of 3 labs ips, treat sickle-cell anemia and ?Parkinsons disease with iPS. Member of National Academy of Sciences, US. * * * HpaII - MspI (CCGG): MspI insensitive to me-C,while HpaII ensitive. and SmaI - XmaI (CCCGGG).? MeDIP: Methyl-DNA immunoprecipitation * H3K4me2: enhancer * In all embryos and somatic cells, high and intermediate level of methylationg tend to occur in low CpG density regions. However, in the oocyte or the pre-implantation embryos, the phenomena of low methylation level tend to occur in high CpG density is not apperent. * 比较每两个连续的阶段中相同片段甲基化程度。用t-假设检验。两个变化阶段，甲基化变化程度有变化的那些片段的变化趋势都是相同的。 * * 反转录转座子(retrotransposon或retroposon)指通过RNA为中介，反转录成DNA后进行转座的可动元件 LINEs: long interspersed elements....(only regions enriched in CpG) L1Md_T and L1Md_Gf closed relative family LTR: long terminal repeat SINEs: short interspersed elements * L1Md_T and L1Md_Gf change most L1Md_A maintain higher methylation in oocyte and zygote LTR: Some change much and some not Red: methylation change 0.45 * Dash line: greatest loss at fertilization IAP(LTR familiy): retention of high methylation * Retrotransposon transcription Bisulophite can’t disinguish mC and hmC, high mC/hmC (retain for several time points) function as methylated * ICR,imprint control region * Dnmt1,Dmnt3b not expression in late oogenesis, possible regulatory function * 2kb promoter region Light grey: CpG Island 附近的一个CpG可以由SNP methylation识别出来自父亲or母亲 X1：父本，C57。1母本 Cleavage=zygote * HCPS: all HC promoters * Group 6 Xiaopeng Ma, Weiru Liu, Zhirui Hu, Weilong Guo About author Alexander Meissner Harvard University Departme