Variances seen in Bacterial Analysis for Water and 细菌分析中的差异.pptVIP

Variances seen in Bacterial Analysis for Water and Waste Water Sampling Gretchen Hathaway Whatman Sales Representative July 19, 2007 Topics M-Endo (LTB, BGB, ECB) M-FC M-FC with rosolic acid MI Broth and Agar ColiCheck ColiCheck with MUG M-Endo M-Endo M-Endo Broth is used for the enumeration of coliforms by membrane filtration. Ideal for drinking water and waste water contaminant testing, M-Endo Coliform Broth is used to differentiate lactose from non-lactose fermenting intestinal organisms. M-Endo : Description M-Endo is a red colored media, which needs to be stored in the dark to prevent discoloration of the media. Gram-positive bacteria are inhibited on this media by the desoxycholate and lauryl sulfate. The addition of ethanol increases the antibacterial nature of the formulation. Lactose fermenting organisms form aldehydes, which react with Schiffs reagent (basic fuchsin and sodium sulfite) to give red colored zones around the colonies. Coliform colonies are therefore red with a characteristic metallic sheen. M-Endo: Interpretation Production of both acid and aldehyde by lactose fermenters, such as Escherichia coli, produce deep red colonies that color the surrounding medium and have a green metallic sheen. Non-lactose fermenters form colorless, translucent colonies M-Endo Verification The chart to the side demonstrates the need of verification steps due to the type of growth. Verification is done in durham tubes with Lauryl Tryptose Broth, Brilliant Green Broth (to confirm Total coliform) and EC Broth (to confirm Fecal coliform). ColiCheck and ColiCheck with MUG ColiCheck and ColiCheck with MUG ColiCheck is used for the presumptive identification of coliforms in water samples by a presence/absence technique. Two product types are available, ColiCheck that identifies coliforms and ColiCheck with MUG that checks for the presence/absence of Escherichia coli. ColiCheck and ColiCheck with MUGDescription Bromocresol purple is a pH indicator which