外周血中钙调蛋白对阿尔茨海默病的诊断价值.docVIP

外周血中钙调蛋白对阿尔茨海默病的诊断价值 　　[摘要] 目的 检测阿尔茨海默病（AD）患者外周血单个核细胞和血浆中钙调蛋白（CaM）的表达水平，评价其作为AD生物标志物的诊断价值。 方法 将2013年2月～2015年1月于青岛大学医学院附属海慈医院就诊和体检的对象共116例纳入本研究，包括AD患者40例、轻度认知障碍（MCI）患者20例、帕金森病（PD）患者20例、额颞叶痴呆（FTD）患者10例、路易体痴呆（DLB）患者10例、进行性核上性麻痹（PSP）患者6例、认知正常的健康体检者（对照组）10名。取外周血分离单个核细胞和血浆，采用Western blot和酶联免疫吸附测定（ELISA）检测CaM在外周血单个核细胞和血浆中的表达水平，并通过绘制ROC曲线判断其诊断能力。 结果 AD患者外周血单个核细胞和血浆中CaM的表达水平显著高于对照组，差异有统计学意义（P 0.05），且CaM的表达水平不随AD的进展变化。以对照组和PSP、DLB、FTD、PD患者中CaM的表达作为标准绘制ROC曲线，曲线下面积分别为0.958、0.946、0.846、0.958和0.896。 结论 CaM在AD患者外周血单个核细胞和血浆中高表达，是一种潜在的AD生物标志物。 　　[关键词] 阿尔茨海默病；钙调蛋白；外周血；生物标志物；诊断价值 　　[中图分类号] R741 [文献标识码] A [文章编号] 1673-7210（2016）01（b）-0050-04 　　[Abstract] Objective To test the expression levels of calmodulin （CaM） in the mononuclear cells and plasma of peripheral blood of patients with Alzheimer disease （AD）， and to evaluate its diagnostic value as the biomarker of AD. Methods Total 116 objects treated and examined in the Affiliated Hiser Hospital of Qingdao University Medical College from February 2013 to January 2015 were enrolled into the research， including 40 cases with AD， 20 cases with mild cognitive impairment （MCI）， 20 cases with Parkinson disease （PD）， 10 cases with frontotemporal dementia （FTD）， 10 cases with dementia with Lewy bodies （DLB）， 6 cases with progressive supranuclear palsy （PSP） and 10 healthy cases with normal cognition （control group）. The peripheral blood was obtained to separate the mononuclear cells and plasma. Western blot and enzyme-linked immuno sorbent assay （ELISA） were used to test the levels of CaM in mononuclear cells and plasma of peripheral blood respectively， and receiver operating characteristic （ROC） curve was drawn to estimate the diagnostic value of CaM. Results Compared with the control group， the levels of CaM in the mononuclear cells and plasma of peripheral blood of patients with AD were significantly higher than those of control group， the differences were statistically significant （P 0.05）， and the expression level of CaM did not