AFP启动子驱动p16基因诱导肝癌细胞系Bel7402的凋亡.pdfVIP

AFP启动子驱动p16基因诱导肝癌细胞系Bel-7402的凋亡 徐丁尧杜芝燕王妍陈惠华徐元基陆应麟幸 【摘要】目的研究p16基因经AFP启动子驱动表达后对肝癌细胞系Bel一7402的作用，探 讨p16基因在肝癌靶向基因治疗中应用的可行性。方法构建了将p16基因置于AFP启动子 驱动的重组复制缺陷型腺病毒Ad-AFP．p16，在重组腺病毒感染肝癌细胞系Beb7402后，应 用Western 因的表达及其对细胞生长、形态及细胞周期的影响。结果感染Ad-AFP-p16的Bel·7402细 胞高表达p16，MTT结果显示细胞生长受到明显抑制，DNA片段化分析和流式细胞术分析 证实p16能诱导肝癌细胞系Bel．7402发生凋亡。结论AFP启动子驱动p16基因表达可以诱 导肝癌细胞系Bel．7402发生凋亡，在肝癌靶向基因治疗中具有潜在的应用前景。 【主题词】p16：甲胎蛋白启动子：细胞凋亡：癌，肝细胞；基因治疗 P16 drivenAFP inducesthe of carcinoma gene hepatocellular by promoter apoptosis Bei-7402cellline．XU Ding—yao，DUZhi—yah，CHENHui-hua，WANGYan，XUYuan-ji，LU of ofBasic of MilitaryAcademy Ying-linDepartmentPathobiology,InstituteMedcine，aeijing Medical 1 Scienses，Beijing00850，China [Abstract] To theeffectof16 drivenAFP onhuman Objectivestudy p gene by promoter carcinomaBel·7402celllineand the useofl6 in hepatocellular explorepotentialp genetargeting for The carcinoma．Methodsrecombination genetherapyhepatocellular replication—deficient Adenovirus 16 the16 drivenAFP WaSconstructedand Ad--AFP·-pcontainingp gene by promoter infected Bel-7402 1 6 was carcinoma cell of detected hepatocellular p gene by line．Expression Western the ladderwereusedtomeaSurethe MTT,flow Blottingassay,and c”ometⅨDNA effects of 16 oncell and cell The